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首页> 外文期刊>Cell biology international. >Caffeine and other xanthines as cytochemical blockers and removers of heterocyclic DNA intercalators from chromatin.
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Caffeine and other xanthines as cytochemical blockers and removers of heterocyclic DNA intercalators from chromatin.

机译：咖啡因和其他黄嘌呤是细胞化学阻滞剂和染色质中杂环DNA嵌入剂的去除剂。

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摘要

Caffeine (CAF) and other xanthines non-covalently bind with the cationic fluorescent dye acridine orange (AO) and with other heterocyclic mutagens and carcinogens that are known to intercalate into double-stranded DNA (dsDNA). Fluorescence microscopy and spectrofluorometry studies were employed to test the ability of caffeine and certain other methyl substituted xanthines, with different binding affinities for AO, to inhibit and to reverse the intercalation of AO and other heterocyclic agents from intercalation with the DNA of nuclear chromatin of air-dried cells. Results indicated that xanthines with binding affinity for AO greater than 150 m(-1) block the AO molecule in a concentration dependent manner and comply with mass action kinetics. Thus CAF and other xanthines can be used to either inhibit intercalation of AO into nuclear DNA or to remove AO once intercalated into nuclear DNA. The interactions between other planar heterocyclics, xanthines, and nuclear chromatin dsDNA were also found to be non-covalent. Studies are needed to determine the ability of CAF and other xanthines to block and/or remove polyaromatic hydrocarbon (PAH) intercalators from the DNA of living cells.
机译：咖啡因（CAF）和其他黄嘌呤与阳离子荧光染料a啶橙（AO）以及与已知插入到双链DNA（dsDNA）中的其他杂环诱变剂和致癌物非共价结合。荧光显微镜和荧光光谱法研究了咖啡因和某些其他甲基取代的黄嘌呤对AO的结合亲和力抑制和逆转AO和其他杂环试剂与空气核染色质DNA的嵌入的能力。干细胞。结果表明，与AO的结合亲和力大于150 m（-1）的黄嘌呤以浓度依赖的方式阻断AO分子并符合质量作用动力学。因此，CAF和其他黄嘌呤可用于抑制AO插入核DNA中或一旦插入AO去除AO。还发现其他平面杂环，黄嘌呤和核染色质dsDNA之间的相互作用是非共价的。需要进行研究以确定CAF和其他黄嘌呤从活细胞的DNA中阻断和/或去除聚芳烃（PAH）嵌入剂的能力。

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