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首页> 外文期刊>Cell biology international. >Morphological and growth alterations in Vero cells transformed by cisplatin.
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Morphological and growth alterations in Vero cells transformed by cisplatin.

机译：顺铂转化的Vero细胞的形态和生长变化。

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摘要

Cisplatin is an antineoplastic agent used to treat solid tumours, such as ovarian, testicular and bladder tumours. However, studies in vitro and in vivo have shown that cisplatin is mutagenic, genotoxic and tumorigenic in other tissues and organs. In this work, we examined the effect of cisplatin on Vero cells, a fibroblast-like cell line. The morphological characteristics were investigated using phase contrast microscopy, scanning electron microscopy and the actin cytoskeleton was labelled with fluorescein isothiocyanate-phalloidin. Cell proliferation was assessed based on the growth curve. Cultured Vero cells treated with cisplatin showed behavioural and morphological alterations associated with cellular transformation. The transformed cells grew in multilayers and formed cellular aggregates. The proliferation and morphological characteristics of the transformed cells were very different from those of control ones. Since transformed Vero cells showed several characteristics related to neoplastic growth, these cells could be a useful model for studying tumour cells in vitro.
机译：顺铂是一种抗肿瘤药，用于治疗实体瘤，例如卵巢癌，睾丸癌和膀胱癌。但是，体外和体内研究表明，顺铂在其他组织和器官中具有致突变性，遗传毒性和致瘤性。在这项工作中，我们检查了顺铂对Vero细胞（一种成纤维细胞样细胞系）的作用。使用相差显微镜，扫描电子显微镜研究形态学特征，并用异硫氰酸荧光素-鬼笔环肽标记肌动蛋白的细胞骨架。基于生长曲线评估细胞增殖。用顺铂处理的培养的Vero细胞显示出与细胞转化有关的行为和形态变化。转化的细胞多层生长并形成细胞聚集体。转化细胞的增殖和形态特征与对照细胞非常不同。由于转化的Vero细胞显示出与肿瘤生长相关的几个特征，因此这些细胞可能成为体外研究肿瘤细胞的有用模型。

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