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首页> 外文期刊>Cell biology international. >Translocation of annexin B1 in response to the stimulation of PMA and ionomycin in cervical cancer cells
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Translocation of annexin B1 in response to the stimulation of PMA and ionomycin in cervical cancer cells

机译：膜联蛋白B1易位对子宫颈癌细胞中PMA和离子霉素的刺激

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摘要

Annexin B1 is a novel member of the annexin superfamily which was isolated from a Cysticercus cellulosae cDNA library. To investigate the physiological roles of annexin B1, we firstly performed immunohistochemical analysis on frozen Cysticercus cellulosae sections and found that annexin B1 was present not only in the tegument of the bladder wall, but also in the host-derived inflammatory layer; In addition, ELISA analysis revealed that annexin B1 could be detected in the cystic fluid of Cysticercus cellulosae and the sera of pigs with cysticercosis. These findings indicated that annexin B1 might be a secretary protein. We further constructed a pEGFP–annexin B1 plasmid and transfected it into SiHa cells. We found that GFP–annexin B1 was stimulated to translocate to the plasma membrane by phorbol 12-myristate 13-acetate (PMA). By contrast, it was induced to distribute at the plasma and nuclear membranes by treatment with calcium ionophore ionomycin. PMA increased annexin B1 membrane binding, which might facilitate exocytosis. Moreover, translocation of the protein to the plasma and nuclear membranes after stimulated by ionomycin, was predicted to be related to an additional function.
机译：膜联蛋白B1是膜联蛋白超家族的新成员，该家族是从Cysticercus cellulosae cDNA文库中分离出来的。为了研究膜联蛋白B1的生理作用，我们首先对冷冻的Cysticercus cellulosae切片进行了免疫组织化学分析，发现膜联蛋白B1不仅存在于膀胱壁的外皮中，而且还存在于宿主衍生的炎症层中。此外，ELISA分析表明，在囊尾Cy和囊尾ice病猪血清中可检测到膜联蛋白B1。这些发现表明膜联蛋白B1可能是秘书蛋白。我们进一步构建了pEGFP–annexin B1质粒，并将其转染到SiHa细胞中。我们发现，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激GFP-annexin B1迁移到质膜。相反，通过用钙离子载体离子霉素处理，诱导其分布在质膜和核膜上。 PMA增加膜联蛋白B1膜结合，这可能促进胞吐作用。此外，预计在离子霉素刺激后，蛋白质向质膜和核膜的易位与另外的功能有关。

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