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首页> 外文期刊>Cell biology international. >Effects of 1alpha,25-dihydroxycholecalciferol and cortisol on the growth and differentiation of primary cultures of mouse mammary epithelial cells in collagen gel.
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Effects of 1alpha,25-dihydroxycholecalciferol and cortisol on the growth and differentiation of primary cultures of mouse mammary epithelial cells in collagen gel.

机译：1alpha，25-dihydroxycholecalciferol和皮质醇对胶原凝胶中小鼠乳腺上皮细胞原代培养物生长和分化的影响。

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We examined the effects of 1alpha,25-dihydroxycholecalciferol (1,25-DHCC) and the glucocorticoid, cortisol, on primary mouse mammary epithelial cells in collagen gel cell culture systems. Physiological low concentrations (10(-11)-10(-9) M) of 1,25-DHCC stimulated growth of the cells in a collagen gel matrix culture in serum-free DMEM+Ham's F12 (1:1) medium containing BSA, EGF and cholera toxin, and the cell number reached 1.8-fold the control after 6 d in culture. In contrast, supraphysiological concentrations (10(-8)-10(-7) M) of 1,25-DHCC suppressed cell growth. Cortisol produced similar, but smaller, dose-dependent effects. The addition of serum to the culture medium masked the stimulatory effect of 1,25-DHCC and both the stimulatory and inhibitory effects of cortisol. 1,25-DHCC also affected casein synthesis by cells cultured in a serum-free floating collagen gel culture containing prolactin, insulin and cortisol, enhancing synthesis at low concentrations (10(-11)-10(-9) M) and inhibiting it above 10(-8) M. In the absence of cortisol, no detectable change in casein synthesis was induced by 1,25-DHCC. These results suggest a physiological role for 1,25-DHCC in stimulating both growth and differentiation of mouse mammary epithelial cells, though 1,25-DHCC does not substitute for glucocorticoids in the differentiation of the cells.
机译：我们检查了胶原凝胶细胞培养系统中的1alpha，25-dihydroxycholecalciferol（1,25-DHCC）和糖皮质激素，皮质醇对原代小鼠乳腺上皮细胞的影响。在不含血清的DMEM + Ham's F12（1：1）含BSA的胶原蛋白凝胶基质培养物中，低浓度（10（-11）-10（-9）M）的1,25-DHCC刺激了细胞的生长，EGF和霍乱毒素，培养6天后细胞数达到对照的1.8倍。相反，1,25-DHCC的超生理浓度（10（-8）-10（-7）M）抑制细胞生长。皮质醇产生相似但较小的剂量依赖性作用。向培养基中添加血清掩盖了1,25-DHCC的刺激作用以及皮质醇的刺激和抑制作用。 1,25-DHCC也影响酪蛋白的合成，该蛋白在含有催乳素，胰岛素和皮质醇的无血清漂浮胶原凝胶培养物中培养，可在低浓度（10（-11）-10（-9）M）时增强合成并抑制它高于10（-8）M。在没有皮质醇的情况下，1,25-DHCC不会引起酪蛋白合成的可检测变化。这些结果表明1,25-DHCC在刺激小鼠乳腺上皮细胞的生长和分化中具有生理作用，尽管1,25-DHCC在细胞分化中不能替代糖皮质激素。

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