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首页> 外文期刊>Cell biology international. >Cell pairing and methylation in Tetrahymena thermophila are altered by exogenous homocysteine.
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Cell pairing and methylation in Tetrahymena thermophila are altered by exogenous homocysteine.

机译：嗜热四膜虫中的细胞配对和甲基化被外源同型半胱氨酸改变。

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摘要

Homocysteine is causally associated with birth defects such as spina bifida, and with premature vascular disease. We have investigated the effects of homocysteine on a cell-cell interaction in a fundamental eukaryotic system, the free-living ciliate Tetrahymena. Exogenously added homocysteine inhibits cell pairing in a dose-dependent manner. These effects are exacerbated by adenosine, which by itself has little demonstrable influence on pairing. S-adenosylhomocysteine (SAH) is a product of the reaction between adenosine and homocysteine, and is an inhibitor of methyl transferases. We therefore predicted that protein methylation would be significantly inhibited by homocysteine. A direct test of that hypothesis involved a demonstration that incorporation of an isotopically labeled methyl group from methionine into proteins was significantly reduced by homocysteine. The undermethylated proteins are of low molecular weight, and might correspond to known methylatable signaling proteins. We show that vanadate, an inhibitor of protein phosphatase, also inhibits cell pairing, and that the effects of vanadate and homocysteine are additive. This is the first demonstration that methylation and possibly phosphorylation play a regulatory role in cell-cell interactions in ciliates.
机译：同型半胱氨酸与出生缺陷（例如脊柱裂）和过早的血管疾病有因果关系。我们已经研究了高半胱氨酸对基本的真核系统，自由生活的纤毛四膜虫细胞-细胞相互作用的影响。外源添加的同型半胱氨酸以剂量依赖的方式抑制细胞配对。腺苷会加剧这些效应，而腺苷本身对配对几乎没有明显的影响。 S-腺苷同型半胱氨酸（SAH）是腺苷和高半胱氨酸之间反应的产物，并且是甲基转移酶的抑制剂。因此，我们预测高半胱氨酸将显着抑制蛋白质甲基化。对这一假说的直接检验涉及一个证明，即高半胱氨酸显着减少了从蛋氨酸向蛋白质中同位素标记的甲基的结合。甲基化不足的蛋白分子量低，可能对应于已知的甲基化信号蛋白。我们显示钒酸盐，一种蛋白磷酸酶的抑制剂，也抑制细胞配对，并且钒酸盐和高半胱氨酸的作用是累加的。这是甲基化和可能的磷酸化在纤毛虫中细胞间相互作用中起调节作用的第一个证明。

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