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首页> 外文期刊>Cell and Tissue Research >Microtubule-associated protein tau (Mapt) is expressed in terminally differentiated odontoblasts and severely down-regulated in morphologically disturbed odontoblasts of Runx2 transgenic mice
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Microtubule-associated protein tau (Mapt) is expressed in terminally differentiated odontoblasts and severely down-regulated in morphologically disturbed odontoblasts of Runx2 transgenic mice

机译：微管相关蛋白tau（Mapt）在Runx2转基因小鼠的终末分化成牙本质细胞中表达，并在形态紊乱的成牙本质细胞中严重下调

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Runx2 is an essential transcription factor for osteoblast and odontoblast differentiation and the terminal differentiation of chondrocytes. We have previously shown that the terminal differentiation of odontoblasts is inhibited in Runx2 transgenic {Tg(Col1a1-Runx2)} mice under the control of the 2.3-kb Col1a1 promoter, which directs the transgene expression to osteoblasts and odontoblasts. Odontoblasts show severe reductions in Dspp and nestin expression and lose their characteristic polarized morphology, including a long process extending to dentin, in Tg(Col1a1-Runx2) mice. We study the molecular mechanism of odontoblast morphogenesis by comparing gene expression in the molars of wild-type and Tg(Col1a1-Runx2) mice, focusing on cytoskeleton-related genes. Using microarray, we found that the gene expression of microtubule-associated protein tau (Mapt), a neuronal phosphoprotein with important roles in neuronal biology and microtubule dynamics and assembly, was high in wild-type molars but severely reduced in Tg(Col1a1-Runx2) molars. Immunohistochemical analysis revealed that Mapt was specifically expressed in terminally differentiated odontoblasts including their processes in wild-type molars but its expression was barely detectable in Tg(Col1a1-Runx2) molars. Doublestaining of Mapt and Runx2 showed their reciprocal expression in odontoblasts. Mapt and tubulin co-localized in odontoblasts in wild-type molars. Immunoelectron microscopic analysis demonstrated Mapt lying around alpha-tubulin-positive filamentous structures in odontoblast processes. Thus, Mapt is a useful marker for terminally differentiated odontoblasts and might play an important role in odontoblast morphogenesis.
机译：Runx2是成骨细胞和成牙本质细胞分化以及软骨细胞终末分化的重要转录因子。我们以前已经表明，在2.3-kb Col1a1启动子的控制下，Runx2转基因{Tg（Col1a1-Runx2）}小鼠中成牙本质细胞的终末分化受到抑制，该启动子将转基因表达导向成骨细胞和成牙本质细胞。成牙本质细胞在Tg（Col1a1-Runx2）小鼠中显示Dspp和nestin表达的严重降低，并失去其特征性的极化形态，包括延伸到牙本质的漫长过程。我们通过比较野生型和Tg（Col1a1-Runx2）小鼠的臼齿中的基因表达来研究成牙本质细胞形态发生的分子机制，重点是细胞骨架相关基因。使用微阵列，我们发现微管相关蛋白tau（Mapt）的基因表达在野生型磨牙中很高，但在Tg（Col1a1-Runx2）中却大大降低，它是在神经元生物学，微管动力学和装配中起重要作用的神经元磷酸化蛋白。 ）磨牙。免疫组织化学分析表明，Mapt在末端分化的成牙本质细胞中特别表达，包括其在野生型磨牙中的过程，但在Tg（Col1a1-Runx2）磨牙中几乎检测不到它的表达。 Mapt和Runx2的双染色表明它们在成牙本质细胞中相互表达。 Mapt和微管蛋白在野生型臼齿的成牙本质细胞中共定位。免疫电子显微镜分析表明，成牙本质细胞过程中Mapt处于α-微管蛋白阳性丝状结构周围。因此，Mapt是终分化成牙本质细胞的有用标记，并且可能在成牙本质细胞形态发生中起重要作用。

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