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首页> 外文期刊>Cell and Tissue Research >Study of the potential spermatogonial stem cell compartment in dogfish testis, Scyliorhinus canicula L.
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Study of the potential spermatogonial stem cell compartment in dogfish testis, Scyliorhinus canicula L.

机译：dog鱼睾丸Scyliorhinus canicula L中潜在的精原干细胞区室的研究。

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摘要

In the lesser-spotted dogfish (Scyliorhinus canicula), spermatogenesis takes place within spermatocysts made up of Sertoli cells associated with stage-synchronized germ cells. As shown in testicular cross sections, cysts radiate in maturational order from the germinative area, where they are formed, to the opposite margin of the testis, where spermiation occurs. In the germinative zone, which is located in a specific area between the tunica albuginea of the testis and the dorsal testicular vessel, individual large spermatogonia are surrounded by elongated somatic cells. The aim of this study has been to define whether these spermatogonia share characteristics with spermatogonial stem cells described in vertebrate and non-vertebrate species. We have studied their ultrastructure and their mitotic activity by 5'-bromo-2'-deoxyuridine (BrdU) incorporation and proliferating cell nuclear antigen (PCNA) immunodetection. Additionally, immunodetection of c-Kit receptor, a marker of differentiating spermatogoniain rodents, and of alpha- and beta-spectrins, as constituents of the spectrosome and the fusome, has been performed. Ultrastructurally, nuclei of stage I spermatogonia present the same mottled aspect in dogfish as undifferentiated spermatogonia nuclei in rodents. Moreover, intercellular bridges are not observed in dogfish spermatogonia, although they are present in stage II spermatogonia. BrdU and PCNA immunodetection underlines their low mitotic activity. The presence of a spectrosome-like structure, a cytological marker of the germline stem cells in Drosophila, has been observed. Our results constitute the first step in the study of spermatogonial stem cells and their niche in the dogfish.
机译：在斑点较少的dog鱼（Scyliorhinus canicula）中，精子发生在由与阶段同步生殖细胞相关的Sertoli细胞组成的精囊内发生。如睾丸横截面所示，囊肿从形成发芽的区域到形成精子的睾丸的相对边缘以成熟顺序放射。在位于睾丸白膜和睾丸背血管之间的特定区域的发芽区，单个大的精原细胞被细长的体细胞包围。这项研究的目的是确定这些精原细胞是否与脊椎动物和非脊椎动物物种中描述的精原干细胞具有共同的特征。我们已经通过5'-溴-2'-脱氧尿苷（BrdU）掺入和增殖细胞核抗原（PCNA）免疫检测研究了它们的超微结构和有丝分裂活性。另外，已经进行了c-Kit受体的免疫检测，c-Kit受体是区分精原性啮齿动物的标志物，以及α-和β-血影蛋白是光谱体和融合体的组成部分。在超微结构中，I型精原细胞核在啮齿类动物中呈现出与未分化的精原细胞核相同的斑驳外观。此外，尽管在二期精子细胞中存在，但在狗鱼精原细胞中未观察到细胞间桥。 BrdU和PCNA免疫检测强调了其低有丝分裂活性。已经观察到果蝇种系干细胞的细胞学标志物即光谱体样结构的存在。我们的结果构成了对白鲑精原干细胞及其生态位研究的第一步。

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