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首页> 外文期刊>Life sciences >Effect of beta-alanyl-L-histidinato zinc on the differentiation of C2C12 cells.
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Effect of beta-alanyl-L-histidinato zinc on the differentiation of C2C12 cells.

机译：β-丙氨酰-L-组氨酸锌对C2C12细胞分化的影响。

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Although beta-alanyl-L-histidinato zinc (AHZ) can promote osteoblast differentiation, the molecular mechanism responsible is not fully understood. The purpose of this study was to determine the effect of AHZ on undifferentiating mesenchymal cells. C2C12, a typical pluripotential mesenchymal cell line, was used. The cells were cultured in 5% serum-containing medium to induce differentiation, either with or without the addition of AHZ. Cell lineage was determined by immunostaining of type II myosin heavy chains, alkaline phosphatase (ALPase) activity, mRNA expression of cellular phenotype-specific markers using semi-quantitative reverse transcriptase-polymerase chain reaction, and core binding factor alpha1/runt-related transcription factor-2 (Cbfa1/Runx2) protein synthesis using Western blot analysis. C2C12 cells cultured in the presence of AHZ were strongly inhibited from developing into myoblasts, and showed high ALPase activity that was approximately double that in the vehicle. The expression of mRNAfor Cbfa1/Runx2, ALPase, Sox9 and type X collagen was increased markedly by the AHZ-stimulated medium, whereas that of desmin and MyoD mRNA was drastically decreased. AHZ increased Cbfa1/Runx2 protein expression substantially. These results provide clear evidence that AHZ converts the differentiation pathway of C2C12 cells to the osteoblast and/or chondroblast lineage.
机译：尽管β-丙氨酰-L-组氨酸锌（AHZ）可以促进成骨细胞分化，但尚不完全清楚其负责的分子机制。这项研究的目的是确定AHZ对未分化的间充质细胞的作用。使用C2C12，一种典型的多能间充质细胞系。在添加或不添加AHZ的情况下，将细胞在含5％血清的培养基中培养以诱导分化。通过使用II型肌球蛋白重链免疫染色，碱性磷酸酶（ALPase）活性，使用半定量逆转录酶-聚合酶链反应的细胞表型特异性标记物的mRNA表达以及核心结合因子alpha1 / runt相关转录因子来确定细胞谱系-2（Cbfa1 / Runx2）蛋白合成，采用蛋白质印迹分析。在AHZ存在下培养的C2C12细胞被强烈抑制发展为成肌细胞，并显示出高ALPase活性，约为载体的两倍。 AHZ刺激的培养基显着增加了Cbfa1 / Runx2，ALPase，Sox9和X型胶原的mRNA表达，而desmin和MyoD mRNA则显着降低。 AHZ大大增加了Cbfa1 / Runx2蛋白表达。这些结果提供了明确的证据，即AHZ将C2C12细胞的分化途径转化为成骨细胞和/或成软骨细胞谱系。

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