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Effects of broad band electromagnetic fields on HSP70 expression and ischemia-reperfusion in rat hearts

机译：宽带电磁场对大鼠心脏HSP70表达和缺血再灌注的影响

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摘要

Although exposure to broad band (0.2-20 MHz) electromagnetic fields (EMF) is part of the treatment of several diseases, little is known as to their effects on myocardial protein expression and resistance to ischemia-reperfusion (I/R). We exposed Sprague-Dawley rats to either high (H, 10 min/day at 200 V/m, 36.1 muT) or low (L, 2 min/day at 30 V/m, 11.4 muT) intensity broad band EMF for 15 days. At the end of the treatment, myocardial HSP70 was 32 +/- 8% (mean +/- SEM) higher in L (P = 0.01) than in control (C), whereas in H it remained the same as in C. Electron microscopy revealed sporadic ruptures of mitochondrial cristae in H hearts, with no differences in other parameters. Malondialdehyde was increased in treated hearts (P < 0.05), but especially in H (P = 0.008). To assess the protective role of HSP70 during I/R, hearts were Langendorff-perfused with Krebs-Henseleit. After I/R, C hearts displayed depressed rate (.) pressure (-13 +/- 7%) and increased end-diastolic (+9.2 +/- 2.8 mmHg) and perfusion pressures (+30 +/- 10 mmHg). In H and L, rate - pressure recovery was similar to C (-2 +/- 21% and -12 +/- 16%, respectively, P = NS). In contrast, both end-diastolic and perfusion pressures were higher in L than in H (30.8 +/- 5.4 vs 18.2 +/- 3.5, P = 0.01, and 54 +/- 8 vs 21 +/- 8 mmHg, P = 0.01, respectively) indicating diastolic derangement in L. In conclusion, the effects of broad band EMF on HSP70 appear to be biphasic, and HSP70 overexpression might not be directly related to improved protection against I/R. (C) 2004 Elsevier Inc. All rights reserved.
机译：尽管暴露于宽带（0.2-20 MHz）电磁场（EMF）是几种疾病的治疗方法，但人们对其对心肌蛋白表达和缺血再灌注抵抗（I / R）的影响知之甚少。我们将Sprague-Dawley大鼠暴露于高强度（H，200 V / m，10分钟/天，36.1 muT）或低强度（L，30 V / m，11.4 muT，2分钟/天）强度15天。在治疗结束时，L组（P = 0.01）的心肌HSP70比对照组（C）高32 +/- 8％（平均+/- SEM），而H组则与C组相同。显微镜检查显示H心脏中线粒体cr的偶发性破裂，其他参数无差异。丙二醛在接受治疗的心脏中增加（P <0.05），但尤其是在H中（P = 0.008）。为了评估HSP70在I / R期间的保护作用，对心脏进行Langendorff灌注Krebs-Henseleit。 I / R后，C心脏显示压低率（。）压力（-13 +/- 7％），舒张末期压力（+9.2 +/- 2.8 mmHg）和灌注压力（+30 +/- 10 mmHg）增加。在H和L中，速率-压力恢复与C相似（分别为-2 +/- 21％和-12 +/- 16％，P = NS）。相比之下，L组的舒张末期压力和灌注压均高于H组（30.8 +/- 5.4 vs 18.2 +/- 3.5，P = 0.01，54 +/- 8 vs 21 +/- 8 mmHg，P = 0.01）分别表示L的舒张功能紊乱。总之，宽带EMF对HSP70的影响似乎是两相的，HSP70的过表达可能与改善的I / R保护作用没有直接关系。 （C）2004 Elsevier Inc.保留所有权利。

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