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首页> 外文期刊>Life sciences >Distribution and immunocytochemical characterization of dorsal root ganglion neurons innervating the lumbar intervertebral disc in rats: A review
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Distribution and immunocytochemical characterization of dorsal root ganglion neurons innervating the lumbar intervertebral disc in rats: A review

机译：支配腰椎间盘的背根神经节神经元的分布和免疫细胞化学特征：综述

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摘要

Previously, it was believed that the lumbar intervertebral disc was innervated segmentally by dorsal root ganglion (DRG) neurons via the sinuvertebral nerves. Recently, it was demonstrated using retrograde tracing methods that the lower disc (L5-L6) is innervated predominantly by upper (L1 and L2) DRG neurons via the sympathetic trunks. Furthermore, we investigated the expression of various pain-related molecules such as calcitonin gene-related peptide (CGRP), isolectin B4 (IB4), P2X(3) receptor and vanniloid receptor 1 (VR1) in DRG neurons innervating the disc using a combination of immunostaining with the retrograde tracing method. This review outlines the distribution and immunocytochemical characterization of DRG neurons innervating the disc. Small nociceptive DRG neurons are classified into nerve growth factor (NGF)-dependent neurons and glial cell line-derived neurotrophic factor (GDNF)-dependent neurons and they can be distinguished by their reactivity for CGRP and 1134, respectively. We found that about half of the neurons innervating the disc were CGRP-immunoreactive (-ir), whilst, only 0.6% of the DRG neurons were IB4-positive, thereby indicating that NGF-dependent neurons are the main subpopulation which transmits and modulates nociceptive information from the disc. In addition, we also demonstrated P2X(3)- and VR1-immunoreactivity in DRG neurons innervating the disc and noted that they were mainly localized in NGF-dependent neurons. It is well known that NGF has sensitizing effects on DRG neurons, with a recent study demonstratng the presence of NGF in the painful intervertebral disc. Therefore, it is suggested that NGF is involved in the generation of discogenic low back pain. (C) 2004 Elsevier Inc. All rights reserved. [References: 113]
机译：以前，人们认为，腰椎间盘是由背根神经节（DRG）神经元经窦神经经节律支配的。最近，使用逆行追踪方法证明，下椎间盘（L5-L6）主要由上（L1和L2）DRG神经元经交感神经干支配。此外，我们研究了各种疼痛相关分子（例如降钙素基因相关肽（CGRP），isolectin B4（IB4），P2X（3）受体和Vanniloid受体1（VR1））在结合使用神经支配盘的DRG神经元中的表达。逆向追踪法进行免疫染色这篇综述概述了支配椎间盘的DRG神经元的分布和免疫细胞化学特征。小型伤害性DRG神经元分为神经生长因子（NGF）依赖性神经元和神经胶质细胞系神经营养因子（GDNF）依赖性神经元，它们可以分别通过其对CGRP和1134的反应性来区分。我们发现，支配椎间盘的神经元中大约有一半是CGRP免疫反应性（-ir），而DRG神经元中只有0.6％的IB4阳性，从而表明NGF依赖性神经元是主要的亚群，可以传递和调节伤害感受性光盘中的信息。此外，我们还证明了DR2神经元分布于椎间盘中的P2X（3）和VR1免疫反应性，并指出它们主要位于NGF依赖性神经元中。众所周知，NGF对DRG神经元具有致敏作用，最近的一项研究表明，在疼痛的椎间盘中存在NGF。因此，建议NGF参与盘源性下腰痛的产生。 （C）2004 Elsevier Inc.保留所有权利。 [参考：113]

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