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首页> 外文期刊>Life sciences >Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats.
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Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats.

机译：动脉粥样硬化大鼠主动脉中血管内皮生长因子和细胞间黏附分子-1表达的时程变化

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摘要

An increasing number of studies have focused on the expressions of growth factors and adhesion molecules in atherosclerotic lesions, which are confirmed to play central roles in angiogenesis and endothelial dysfunction, including vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1). However, the difference of growth factor and adhesion molecule expression time courses has not been determined in vivo. This study aimed to determine the expression patterns and expression curves of ICAM-1 and VEGF in atherosclerotic rats during the time course. An experiment atherosclerotic model in rats was established by combining the high fat/cholesterol diets with injection of vitamin D3. In situ hybridization was used to determine the expression patterns of VEGF and ICAM-1 in aortas of normal or atherosclerotic rats in 8 weeks. There was a massive increase in reactivity for both ICAM-1 and VEGF in atherosclerotic plaques. Northern blot, Western blot and ELISA analysis were used to quantify VEGF and ICAM-1 expressions in time course. In rat aorta, the expression curves in time course showed that ICAM-1, not VEGF, was up-regulated in mRNA levels significantly in 2 weeks; while VEGF expression was hysteresis than ICAM-1, which showed maximum expression level in 8 weeks. Our results provide the evidence of VEGF and ICAM-1 expression curves in time course in atherosclerotic rats, which indicated different regulatory mechanisms of VEGF and ICAM-1 expression in atherogenesis.
机译：越来越多的研究集中于动脉粥样硬化病变中生长因子和粘附分子的表达，这些被证实在血管生成和内皮功能障碍中起着核心作用，包括血管内皮生长因子（VEGF）和细胞间粘附分子-1（ICAM- 1）。但是，尚未在体内确定生长因子和粘附分子表达时间进程的差异。本研究旨在确定动脉粥样硬化大鼠在一段时间内ICAM-1和VEGF的表达模式和表达曲线。通过将高脂/胆固醇饮食与注射维生素D3结合来建立大鼠实验性动脉粥样硬化模型。原位杂交用于确定正常和动脉粥样硬化大鼠在8周内VEGF和ICAM-1在主动脉中的表达模式。在动脉粥样硬化斑块中，ICAM-1和VEGF的反应性都大大增加。用Northern印迹，Western印迹和ELISA分析定量测定VEGF和ICAM-1的表达。在大鼠主动脉中，随时间变化的表达曲线表明，ICAM-1而不是VEGF在2周内显着上调了mRNA水平。 VEGF的表达比ICAM-1有滞后性，在8周内达到最大表达水平。我们的结果提供了动脉粥样硬化大鼠中VEGF和ICAM-1表达曲线随时间变化的证据，表明了动脉粥样硬化中VEGF和ICAM-1表达的不同调节机制。

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