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首页> 外文期刊>Life sciences >Gluten exorphin B5 stimulates prolactin secretion through opioid receptors located outside the blood-brain barrier.
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Gluten exorphin B5 stimulates prolactin secretion through opioid receptors located outside the blood-brain barrier.

机译：面筋外啡肽B5通过位于血脑屏障外部的阿片受体刺激催乳激素分泌。

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摘要

Gluten exorphin B5 (GE-B5) is a food-derived opioid peptide identified in digests of wheat gluten. We have recently shown that GE-B5 stimulates prolactin (PRL) secretion in rats; this effect is abolished by preadministration of the opioid receptor antagonist naloxone. However, since the structure of naloxone allows it to cross the blood-brain barrier (BBB) and antagonize opioid effects centrally as well as peripherally, it could not established, on the basis of those data, if GE-B5-induced PRL release is exerted through sites located inside or outside the BBB. In this study, we sought to determine the site of action of GE-B5 on PRL secretion, by pretreating male rats with naloxone methobromide (NMB), an opioid antagonist that does not cross the BBB. Four groups of rats were given the following treatments: 1) intravenous vehicle; 2) intravenous GE-B5 (3 mg kg(-1) body weight); 3) intraperitoneal NMB (5 mg kg(-1) body weight), followed by vehicle; 4) NMB, followed by GE-B5. Blood samples for PRL were taken at intervals for 40 minutes after vehicle or GE-B5 administration. GE-B5 stimulated PRL secretion; the effect was statistically significant at time 20. NMB preadministration completely abolished PRL response. Our experiment indicates that GE-B5 stimulates PRL secretion through opioid receptors located outside the BBB. Since opioid peptides do not exert their effect on PRL secretion directly, but via a reduced dopaminergic tone, our data suggest that GE-B5 can modify brain neurotransmitter release without crossing the BBB.
机译：麸质外啡肽B5（GE-B5）是一种在小麦面筋消化物中鉴定出的食品类阿片肽。我们最近发现，GE-B5刺激大鼠催乳激素（PRL）的分泌。预先服用阿片受体拮抗剂纳洛酮可消除这种作用。但是，由于纳洛酮的结构使其能够穿过血脑屏障（BBB）并在中央和外围拮抗阿片类药物的作用，因此根据这些数据，如果GE-B5诱导的PRL释放是通过位于BBB内部或外部的站点施加。在这项研究中，我们试图通过用不穿越BBB的阿片类拮抗剂纳洛酮（NMB）预处理雄性大鼠，确定GE-B5对PRL分泌的作用部位。四组大鼠接受以下治疗：1）静脉内溶媒; 2）静脉注射GE-B5（3 mg kg（-1）体重）; 3）腹膜内NMB（5 mg kg（-1）体重），其次是媒介物; 4）NMB，然后是GE-B5。在施用媒介物或GE-B5后，间隔40分钟采集PRL的血液样品。 GE-B5刺激PRL分泌;该效果在时间20时具有统计学意义。NMB预先给药完全取消了PRL反应。我们的实验表明，GE-B5通过位于BBB外的阿片受体刺激PRL分泌。由于阿片样物质肽并不直接对PRL分泌发挥作用，而是通过降低的多巴胺能基调发挥作用，因此我们的数据表明GE-B5可以修饰脑神经递质释放而不会越过BBB。

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