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Stress-induced, breakdown of intestinal barrier function in the rat: Reversal by wood creosote

机译：应激诱导的大鼠肠屏障功能破坏：木杂酚酸酯逆转

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摘要

Our previous studies demonstrated that wood creosote (Seirogan) inhibits intestinal secretion and normalizes the transport of electrolytes and water in rats subjected to restraint stress. The goal of the present study was to examine whether wood creosote has a protective effect against stress-induced breakdown of intestinal barrier function. F-344 rats were subjected to 90-min water avoidance stress (WAS) with wood creosote (30 mg/kg) or vehicle administered intragastrically 30 min prior to WAS. Sham stressed rats received wood creosote or vehicle treatment but did not experience the WAS. All rats were euthanized at the end of the WAS or sham-stress and the jejunum and colon were isolated. Epithelial transport was studied in modified Ussing chambers. Spontaneous secretion was assessed by electrophysiological measurement of the short circuit current (I-sc) while electrical conductance (G) was calculated from the potential difference (PD) and I-sc using Ohm's law. Intestinal permeability was defined by the mucosal-to-serosal flux of horseradish peroxidase (HRP). WAS significantly elevated basal I-sc and G and increased epithelial permeability to HRP in the jejunum but not in the colon. Wood creosote resulted in a significant reduction of the stress-induced increase in I-sc, G and the mucosal-to-serosal flux of HRP compared to the vehicle-treated group. Wood creosote caused no significant effects in sham-stressed rats. The results suggest that oral administration of wood creosote may prevent stress-induced diarrhea by preventing aversive effects on small intestinal secretion and barrier function. (c) 2006 Elsevier Inc. All rights reserved.
机译：我们以前的研究表明，木杂酚油（Seirogan）抑制肠道分泌，并使处于束缚应激状态的大鼠体内的电解质和水正常化。本研究的目的是研究木材杂酚油是否对应激诱导的肠屏障功能破坏具有保护作用。 F-344大鼠在使用木杂酚油（30 mg / kg）或在灌洗前30分钟通过胃内给药接受90分钟的避水应激（WAS）。 Sham强调大鼠接受木杂酚油或媒介物处理，但未经历WAS。在WAS结束时对所有大鼠实施安乐死或假手术，并分离空肠和结肠。上皮运输在改良的Ussing室进行了研究。通过短路电流（I-sc）的电生理测量来评估自发分泌，同时使用欧姆定律根据电势差（PD）和I-sc计算电导率（G）。肠渗透性由辣根过氧化物酶（HRP）的粘膜-浆液通量定义。 WAS在空肠中显着升高了基础I-sc和G，并增加了对空肠HRP的上皮通透性，但在结肠中则不然。与媒介物处理组相比，木杂酚油可显着降低应激诱导的HRP的I-sc，G和黏膜-浆液通量的增加。木材杂酚油对假应激大鼠无明显影响。结果表明，口服木杂酚油可通过防止对小肠分泌物和屏障功能的反作用来预防应激性腹泻。 （c）2006 Elsevier Inc.保留所有权利。

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