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首页> 外文期刊>Life sciences >Effect of sinomenine on gene expression of the IL-1 beta-activated human synovial sarcoma
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Effect of sinomenine on gene expression of the IL-1 beta-activated human synovial sarcoma

机译：青藤碱对IL-1β激活的人滑膜肉瘤基因表达的影响

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摘要

Sinomenine is an alkaloid with pharmacological effects of anti-inflammation. anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1 beta. The proliferative effect of sinomenine was examined in the presence or absence of IL-1 beta by the [H-3]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1 beta and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1 beta could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1 beta-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PIGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma. (c) 2006 Elsevier Inc. All rights reserved.
机译：青藤碱是一种具有抗炎药理作用的生物碱。抗血管生成，抗关节炎和免疫抑制。这项研究旨在研究青藤碱对IL-1β激活的人滑膜肉瘤细胞（Hs701.T）基因表达的影响。通过[H-3]-胸苷掺入法和MTT法分别检测在有无IL-1β存在下青藤碱的增殖作用。利用DNA微阵列技术和RT-PCR，同时测定IL-1β的激活作用和青藤碱对Hs701.T的调节作用。结果表明，IL-1β可以刺激Hs701.T细胞的增殖和基因表达。青藤碱可以显着抑制IL-1β激活的Hs701.T细胞的增殖，并抑制IL-6，PIGF，Daxx和HSP27等17个基因的表达。通过炎症，细胞粘附，增殖，凋亡和血管生成的介导，发现这些基因在肿瘤进展中很重要。总之，我们的研究为青藤碱对滑膜肉瘤的药理作用的进一步研究提供了补充信息。 （c）2006 Elsevier Inc.保留所有权利。

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