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Effects of compressive force on the differentiation of pluripotent mesenchymal cells

机译：压力对多能间充质细胞分化的影响

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The purpose of this study was to determine the effect of mechanical stress on the differentiation of the pluripotent mesenchymal cell line C2C12. C2C12 cells were cultured continuously under compressive force (0.25-2.0 g/cm(2)). After mechanical stress loading, the levels of expression of mRNAs and proteins for phenotype-specific markers of osteoblasts (Runx2, Msx2, Dlx5, Osterix, AJ18), chondroblasts (Sox5, Sox9), myoblasts (MyoD), and adipocytes (PPAR gamma) were measured by real-time polymerase chain reaction analysis and Western blot analysis, respectively. The expression of activated p38 mitogen-activated protein kinase (p38 MAPK) was measured by Western blotting and/or ELISA. Loading 0.5 g/cm(2) of compressive force significantly increased the expression levels of Runx2, Msx2, Dlx5, Osterix, Sox5, and Sox9. In contrast, the expression levels of AJ18, MyoD, and PPAR gamma were decreased by exposure to 0.5 g/cm(2) of compressive force. Loading 0.5 g/cm(2) of compressive force also induced the phosphorylation of p38 MAPK. SB203580, which is a specific inhibitor of p38 MAPK, inhibited the compressive force-induced phosphorylation of p38 MAPK and partially blocked compressive force-induced Runx2 mRNA expression. These results demonstrate that compressive force stimulation directs the differentiation pathway of C2C12 cells into the osteoblast and chondroblast lineage via activated phosphorylation of p38 MAPK. (c) 2007 Elsevier Inc. All rights reserved.
机译：这项研究的目的是确定机械应力对多能间充质细胞系C2C12分化的影响。 C2C12细胞在压力（0.25-2.0 g / cm（2））下连续培养。机械应力加载后，成骨细胞（Runx2，Msx2，Dlx5，Osterix，AJ18），成软骨细胞（Sox5，Sox9），成肌细胞（MyoD）和脂肪细胞（PPARγ）的表型特异性标记的mRNA和蛋白质的表达水平分别通过实时聚合酶链反应分析和蛋白质印迹分析测量。通过Western印迹和/或ELISA测量活化的p38促丝裂原活化蛋白激酶（p38 MAPK）的表达。加载0.5 g / cm（2）的压缩力显着提高了Runx2，Msx2，Dlx5，Osterix，Sox5和Sox9的表达水平。相比之下，AJ18，MyoD和PPARγ的表达水平通过暴露于0.5 g / cm（2）的压缩力而降低。加载0.5 g / cm（2）的压缩力也诱导了p38 MAPK的磷酸化。 SB203580是p38 MAPK的特异性抑制剂，可抑制压力诱导的p38 MAPK磷酸化并部分阻断压力诱导的Runx2 mRNA表达。这些结果表明，压力刺激通过p38 MAPK的活化磷酸化将C2C12细胞的分化途径引导到成骨细胞和成软骨细胞谱系中。 （c）2007 Elsevier Inc.保留所有权利。

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