• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Expression, microsomal and mitochondrial activities of cytochrome P450 enzymes in brain regions from control and phenobarbital-treated rabbits.
【24h】

Expression, microsomal and mitochondrial activities of cytochrome P450 enzymes in brain regions from control and phenobarbital-treated rabbits.

机译：对照和苯巴比妥治疗的兔脑区域中细胞色素P450酶的表达，微粒体和线粒体活性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression and monooxygenase activity of various cytochrome P450 (CYP) enzymes along with constitutive androstane (CAR) and the pregnane X (PXR) receptors were investigated in the brain of control and phenobarbital-treated rabbits (80 mg/kg for 4 days). RT-PCR analysis, using specific primers, demonstrated that in control rabbits mRNAs of CYP 2A10, 2B4/5 and 3A6 were expressed, though to a different extent, in the liver, as well as in brain cortex, midbrain, cerebellum, striatum, hippocampus and hypothalamus, whilst CYP2A11 and 4B1 were not expressed in the hypothalamus. CAR was expressed in liver and all the brain regions examined, whereas the PXR was expressed only in liver and cortex. Real time RT-PCR analysis demonstrated that in vivo treatment with phenobarbital, in contrast with what happened in liver, did not induce the expression of CYP 2B4/5 mRNA in cortex, midbrain and cerebellum. NADPH cytochrome c reductase and some other enzymatic activities markers of CYP 2A, 2B, 3A and 4B activities werestudied in liver microsomes as well as in microsomes and mitochondria of brain cortex, midbrain and cerebellum of control and phenobarbital-treated rabbits. In contrast to what was observed in liver, phenobarbital treatment did not induce the aforementioned monooxygenase activities in brain. However, we cannot exclude that a longer phenobarbital treatment may lead to a significant induction of CYP activities in brain. These findings indicated that brain CYPs, despite the presence of CAR, were resistant to phenobarbital induction, indicating a possible different regulation of these enzymes between brain and liver.
机译：在对照和苯巴比妥治疗的兔子（80 mg / kg，4天）的大脑中研究了各种细胞色素P450（CYP）酶以及组成型雄激素（CAR）和孕烷X（PXR）受体的表达和单加氧酶活性。使用特异性引物进行的RT-PCR分析表明，在对照组兔子中，CYP 2A10、2B4 / 5和3A6的mRNA在肝脏以及大脑皮质，中脑，小脑，纹状体中均有不同程度的表达，海马和下丘脑，而CYP2A11和4B1在下丘脑中不表达。 CAR在肝脏和所有检查的大脑区域中表达，而PXR仅在肝脏和皮质中表达。实时逆转录-聚合酶链反应（PCR）分析表明，苯巴比妥的体内治疗与肝脏发生的情况相反，并未诱导CYP 2B4 / 5 mRNA在皮质，中脑和小脑中的表达。研究了肝脏微粒体以及对照和苯巴比妥治疗的兔的大脑皮层，中脑和小脑的微粒体和线粒体的NADPH细胞色素C还原酶和其他一些酶活性的CYP 2A，2B，3A和4B活性标记。与在肝脏中观察到的相反，苯巴比妥治疗并未在大脑中诱导上述单加氧酶活性。但是，我们不能排除更长的苯巴比妥治疗可能导致大脑中CYP活性的明显诱导。这些发现表明，尽管存在CAR，但脑CYP对苯巴比妥的诱导仍具有抗性，表明在脑和肝之间可能对这些酶进行了不同的调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号