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Genetic engineering of mammals

机译:哺乳动物的基因工程

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Historically, genetic engineering for mammalian reproductive questions has been accomplished primarily in the mouse. However, all the genetic manipulations that can be done in the mouse can now be accomplished in most domesticated mammals. Random integration of transgenes, homologous recombination and gene editing are now routine for several mammalian species. For livestock, queries related to fertility can be asked directly for the species in question, without a need for a mouse model. For human clinical concerns, the most appropriate model should be selected based on physiology, anatomy, or even size. The mouse will continue to be a useful genetically engineered model. However, other species are now amenable to the full range of genetic manipulations and should be considered as possible models for human conditions when appropriate.
机译:从历史上看,哺乳动物生殖问题的基因工程主要是在小鼠中完成的。但是,现在可以在大多数驯养的哺乳动物中完成所有可以在小鼠中进行的遗传操作。现在,转基因的随机整合,同源重组和基因编辑对于几种哺乳动物都是常规的。对于牲畜,可以直接询问所涉及的物种的与繁殖力有关的查询,而无需使用鼠标模型。对于人类的临床问题,应根据生理,解剖甚至大小来选择最合适的模型。鼠标将继续是有用的基因工程模型。但是,其他物种现在也适用于所有种类的遗传操作,并在适当时应考虑为可能的人类条件模型。

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