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首页> 外文期刊>Life sciences >TISSUE CULTURE SUPERNATANTS FROM HUMAN ISLETS OF LANGERHANS ACTIVATE THE OXIDATIVE BURST RESPONSE OF HUMAN MONOCYTES IN VITRO
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TISSUE CULTURE SUPERNATANTS FROM HUMAN ISLETS OF LANGERHANS ACTIVATE THE OXIDATIVE BURST RESPONSE OF HUMAN MONOCYTES IN VITRO

机译：朗格汉斯人胰岛组织培养上清激活人单核细胞的氧化爆发响应。

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Macrophages play a major role in the pathogenesis of insulin-dependent diabetes mellitus in animals. These cells are the first to invade the pancreas and macrophage-eradicating treatments reduce the incidence of the disease. Tn humans, however, their role is less clear. In this study we investigated the hypothesis that the pancreatic environment per se could activate macrophages. Tissue culture supernatants from human islets of Langerhans were tested for chemotactic activity and oxidative burst response in monocytes isolated from healthy adults. Preincubation with the supernatants enhanced the oxidative burst response evoked by fMLP (up to 379%) and opsonized zymosan (up to 173%). The activity decreased by dilution and was no longer detectable at 1:16. No increased activity was seen in supernatants from a number of other human endocrine and non-endocrine primary cells, suggesting a factor specific for islet tissue. The increased oxidative burst response could partially be eliminated by heat- and proteinase K treatment, suggesting that the activity could be of polypeptide nature. The factor could not be absorbed by polyvalent rabbit antibodies directed towards a variety of cytokines nor by a mixture of high-titer anti-cytokine antibodies. It is possible that islet factors could also promote such monocyte activation in vivo in monocytes attracted to the islets of Langerhans by other means. This could contribute to the development of insulin-dependent diabetes in humans. [References: 42]
机译：巨噬细胞在动物中胰岛素依赖型糖尿病的发病机理中起主要作用。这些细胞是最早侵袭胰腺的细胞，根除巨噬细胞的治疗可降低疾病的发病率。然而，在人类中，他们的作用还不清楚。在这项研究中，我们研究了胰腺环境本身可以激活巨噬细胞的假说。测试了来自郎格罕氏人胰岛的组织培养上清液在从健康成年人中分离出的单核细胞中的趋化活性和氧化爆发反应。与上清液的预孵育增强了fMLP（高达379％）和调理过的酵母聚糖（高达173％）引起的氧化爆发反应。稀释后活性降低，并且在1:16不再可检测到。在许多其他人的内分泌和非内分泌原代细胞的上清液中未见活性增加，提示胰岛组织特有的因子。通过加热和蛋白酶K处理可以部分消除增加的氧化猝发反应，表明该活性可能具有多肽性质。该因子不能被针对多种细胞因子的多价兔抗体吸收，也不能被高滴度抗细胞因子抗体的混合物吸收。胰岛因子也可能在通过其他方式吸引朗格罕氏岛的单核细胞中在体内促进这种单核细胞活化。这可能有助于人类胰岛素依赖型糖尿病的发展。 [参考：42]

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