IDENTIFICATION OF RAT URINARY KININ AS BRADYKININ

Hagiwara Y.; Kuraishi T.; Hayashi I.; Miyata T.; Ohishi S.; Kojima M.

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IDENTIFICATION OF RAT URINARY KININ AS BRADYKININ

机译：大鼠尿激肽缓激肽的鉴定

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Bradykinin (BK)-like activity, which was detected by BK-enzyme-immunoassay, was purified from 80 ml of ureter urine of Sprague-Dawley rats by Sephadex G 25 chromatography, FPLC, and reversed phase HPLC. The purified kinin fraction showed the same retention time as authentic BK on HPLC and produced contraction of isolated rat uterus, the contraction being suppressed by a B2-antagonist Hoe140. There was no other kinin detected on the HPLC at the corresponding retention time to kallidin, arginyl-BK or T-kinin. The peptide showed an amino acid sequence identical to that of BK by amino acid sequence analysis. [References: 16]

机译：通过Sephadex G 25色谱，FPLC和反相HPLC从80 ml的Sprague-Dawley大鼠输尿管尿液中纯化出通过BK酶免疫测定法检测的类似缓激肽（BK）的活性。纯化的激肽级分在HPLC上显示与真实BK相同的保留时间，并产生离体大鼠子宫的收缩，该收缩被B2拮抗剂Hoe140抑制。在对应保留时间的HPLC上，未检测到针对激肽释放酶，精氨酰-BK或T-激肽的其他激肽。通过氨基酸序列分析，该肽显示出与BK相同的氨基酸序列。 [参考：16]

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《Life sciences》 |1995年第10期|共6页
作者
Hagiwara Y.; Kuraishi T.; Hayashi I.; Miyata T.; Ohishi S.; Kojima M.;
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正文语种 eng
中图分类生命的起源;生命科学总论;
关键词
Bradykinin; Urine; Ureter urine; T-kinin; Excretion;

机译：缓激肽;尿;尿尿;T-激肽;排泄;

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专利

1. IDENTIFICATION OF RAT URINARY KININ AS BRADYKININ [J] . Hagiwara Y., Kuraishi T., Hayashi I., Life sciences . 1995,第10期

机译：大鼠尿激肽缓激肽的鉴定
2. EXISTENCE OF A BRADYKININ-LIKE SUBSTANCE, AND INFLUENCE OF CARBACHOL AND ATROPINE ON KININ-FORMING AND DESTROYING ACTIVITIES, AND KININ AND KININOGEN CONTENTS IN RAT STOMACH [J] . Katsuya OHATA, Motoki KOBAYASHI, Sadao MIYATA, Japanese Journal of Pharmacology . 1980,第5期

机译：缓激肽样物质的存在以及卡巴酚和阿托品对大鼠气孔激肽形成和破坏活性以及激肽和激肽原含量的影响
3. EXISTENCE OF A BRADYKININ-LIKE SUBSTANCE, AND INFLUENCE OF CARBACHOL AND ATROPINE ON KININ-FORMING AND DESTROYING ACTIVITIES, AND KININ AND KININOGEN CONTENTS IN RAT STOMACH [J] . Katsuya OHATA, Motoki KOBAYASHI, Sadao MIYATA, Japanese Journal of Pharmacology . 1980,第5期

机译：缓激肽样物质的存在以及卡巴酚和阿托品对大鼠气孔激肽形成和破坏活性以及激肽和激肽原含量的影响
4. Identification of urinary pathogens with species-specific 16s rDNA probes under rapid detection time at ambient temperature for the development of a MEMsbased urinary pathogen DNA biosensor [C] . Chien-Pin Sun, Joseph C. Liao, Yao-Hua Zhang, The 2nd World Congress for Chinese Biomedical Engineers . 2004

机译：在室温下快速检测时间下，用物种特异性的16s rDNA探针鉴定尿中病原体，以开发基于MEMs的尿中病原体DNA生物传感器
5. Contribution of tachykinin and kinin receptors in central autonomic control of blood pressure and behavioural activity in hypertensive rats. [D] . Pereira, Helaine De Brito. 2010

机译：速激肽和激肽受体在高血压大鼠中央自主控制血压和行为活动中的作用。
6. The kinin system - bradykinin: biological effects and clinical implications. Multiple role of the kinin system - bradykinin [O] . Ch Golias, A Charalabopoulos, D Stagikas, 2007

机译：激肽系统-缓激肽：生物学效应和临床意义。激肽系统的多重作用-缓激肽
7. Ile-Ser-bradykinin (T-kinin) and Met-Ile-Ser-bradykinin (Met-T-kinin) are released from T-kininogen by an acid proteinase of granulomatous tissues in rats [O] . Sakamoto Wataru, Satoh Fumihiko, Gotoh Katsuhiro, 1987

机译：大鼠肉芽肿组织的酸性蛋白酶从T-激肽原中释放出Ile-Ser-缓激肽（T-激肽）和Met-Ile-Ser-缓激肽（Met-T-激肽）
8. Identification of lle-Ser-Bradykinin in Aqueous Samples by Liquid Chromatographywith Ultraviolet and Electrospray Mass Spectrometric Detection [R] . Hancock, J. R., D'Agostino, P. A., Provost, L. R. 1995

机译：液相色谱 - 紫外和电喷雾质谱检测水样中Lle-ser-Bradykinin的鉴定

IDENTIFICATION OF RAT URINARY KININ AS BRADYKININ

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