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首页> 外文期刊>Cell and Tissue Research >Expression studies of neuronatin in prenatal and postnatal rat pituitary
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Expression studies of neuronatin in prenatal and postnatal rat pituitary

机译：神经素在产前和产后大鼠垂体中的表达研究

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摘要

The pituitary gland, an indispensable endocrine organ that synthesizes and secretes pituitary hormones, develops with the support of many factors. Among them, neuronatin (NNAT), which was discovered in the neonatal mouse brain as a factor involved in neural development, has subsequently been revealed to be coded by an abundantly expressing gene in the pituitary gland but its role remains elusive. We analyze the expression profile of Nnat and the localization of its product during rat pituitary development. The level of Nnat expression was high during the embryonic period but remarkably decreased after birth. Immunohistochemistry demonstrated that NNAT appeared in the SOX2-positive stem/progenitor cells in the developing pituitary primordium on rat embryonic day 11.5 (E11.5) and later in the majority of SOX2/PROP1 double-positive cells on E13.5. Thereafter, during pituitary embryonic development, Nnat expression was observed in some stem/progenitor cells, proliferating cells and terminally differentiating cells. In postnatal pituitaries, NNAT-positive cells decreased in number, with most coexpressing Sox2 or Pit1, suggesting a similar role for NNAT to that during the embryonic period. NNAT was widely localized in mitochondria, peroxisomes and lysosomes, in addition to the endoplasmic reticulum but not in the Golgi. The present study thus demonstrated the variability in expression of NNAT-positive cells in rat embryonic and postnatal pituitaries and the intracellular localization of NNAT. Further investigations to obtain functional evidence for NNAT are a prerequisite.
机译：垂体腺是合成和分泌垂体激素必不可少的内分泌器官，它在许多因素的支持下得以发展。其中，在新生小鼠大脑中发现的神经素（NNAT）是神经发育的一个相关因素，随后被发现由垂体中大量表达的基因编码，但其作用仍然难以捉摸。我们分析了Nnat在大鼠垂体发育过程中的表达谱及其产物的定位。 Nnat表达水平在胚胎期高，但出生后明显下降。免疫组织化学表明，在大鼠胚胎第11.5天（E11.5），NNAT出现在发育中的垂体原基中的SOX2阳性干/祖细胞中，随后在E13.5的大多数SOX2 / PROP1双阳性细胞中出现。此后，在垂体胚胎发育过程中，在某些干/祖细胞，增殖细胞和终末分化细胞中观察到Nnat表达。在产后垂体中，NNAT阳性细胞数量减少，大多数共表达Sox2或Pit1，表明NNAT的作用与胚胎期相似。除了内质网外，NNAT广泛分布于线粒体，过氧化物酶体和溶酶体中，但不在高尔基体中。因此，本研究证明了大鼠胚胎和出生后垂体中NNAT阳性细胞表达的可变性以及NNAT的细胞内定位。进行进一步研究以获取NNAT的功能证据是前提。

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