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首页> 外文期刊>Life sciences >Effect of crocin on experimental atherosclerosis in quails and its mechanisms.
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Effect of crocin on experimental atherosclerosis in quails and its mechanisms.

机译：番红花对鹌鹑实验性动脉粥样硬化的作用及其机制。

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摘要

In the present study, we examined the prophylaxis effect of crocin on experimental atherosclerosis and its possible mechanisms. The atherosclerosis formation was induced by hyperlipidamic diet in quails. At the 9th week, serum lipid, MDA and NO were measured, and HE staining was used to investigate the histopathological changes of aorta. Bovine aortic endothelial cells (EC) were obtained from the thoracic aorta of newborn calves. After incubation of the cells with Ox-LDL (50 mg.L(-) (1)) for 24 h, the activities of LDH, NO in culture media and activity of NOS in endothelial cells were measured, flow cytometer was used to determine the rate of endothelial cells apoptosis. Peritoneal macrophages were obtained from thioglycolate-injected mice. Cholesterol and free cholesterol in cells were assayed after incubation of the cells with Ox-LDL. Bovine aortic smooth muscle cells (SMC) were obtained from the thoracic aorta of newborn calf. Proliferation was induced by 100 mug.L(-) (1) Ox-LDL and antiproliferative effect of crocin on SMCs were observed. SMCs cycle phases were measured by flow cytometry. SMCs were loaded with Fluo-3/AM and [Ca(2+)](i) was measured by Laser Scanning Confocal Microscope (LSCM). Crocin could reduce the level of serum TC, TG, LDL-C and inhibit the formation of aortic plaque. Crocin could reduce MDA and inhibit the descending of NO in serum. Compared with control, Ox-LDL group could increase the activity of LDH and decrease activity of NO in culture media and activity of NOS in endothelial cells, preincubated with crocin, the effects of Ox-LDL were inhibited. Crocin could decrease the EC apoptosis induced by Ox-LDL. Crocin concentration-dependently inhibited the TC and CE elevation induced by Ox-LDL in macrophages. Crocin could inhibit the proliferation of SMCs induced by Ox-LDL. In the presence or absence of extracellular Ca(2+), crocin concentration-dependently inhibited the [Ca(2+)](i) elevation induced by 120mg.L(-) (1)Ox-LDL, In the absence of extracellular Ca(2+), crocin could inhibit the [Ca(2+)](i) elevation induced by CHCl(3) in a concentration-dependent manner. The results indicated that crocin could inhibit the formation of atherosclerosis in quails. Crocin had protective effects on endothelial cells. Crocin could decrease CE in macrophages and uptake of Ox-LDL, inhibiting the formation of foam cell, which would promote the initiation and progression of atherosclerosis. Crocin could inhibit the [Ca(2+)](i) elevation in smooth muscle cell, Ca(2+) is an important second messenger that regulates a variety of cellular processes, including smooth muscle cell proliferation and gene expression . Crocin exerted antiatherosclerotic effects through decreasing the level of Ox-LDL that plays an important role in the initiation and progression of atherosclerosis.
机译：在本研究中，我们研究了番红花对实验性动脉粥样硬化的预防作用及其可能的机制。高脂饮食在鹌鹑中诱导了动脉粥样硬化的形成。在第9周，测量血脂，MDA和NO，并且HE染色用于调查主动脉的组织病理学变化。牛主动脉内皮细胞（EC）从新生小牛的胸主动脉获得。将细胞与Ox-LDL（50 mg.L（-）（1））孵育24小时后，测量培养基中LDH，NO的活性和内皮细胞中NOS的活性，使用流式细胞仪确定内皮细胞凋亡率。从注射巯基乙酸盐的小鼠获得腹膜巨噬细胞。用Ox-LDL孵育细胞后，测定细胞中的胆固醇和游离胆固醇。牛主动脉平滑肌细胞（SMC）从新生小腿的胸主动脉获得。 100μg/ L诱导增殖。L（-）（1）Ox-LDL和番红花对SMC具有抗增殖作用。通过流式细胞术测量SMC的周期阶段。 SMC装有Fluo-3 / AM，并通过激光扫描共聚焦显微镜（LSCM）测量[Ca（2 +）]（i）。番红花可以降低血清TC，TG，LDL-C水平并抑制主动脉斑块的形成。番红花可以降低MDA并抑制NO的下降。与对照组相比，Ox-LDL组与罗红素预孵育后，可增加培养基中LDH的活性，降低培养基中NO的活性和内皮细胞中NOS的活性，从而抑制了Ox-LDL的作用。番红花可以减少Ox-LDL诱导的EC凋亡。番红花浓度依赖性地抑制了巨噬细胞中Ox-LDL诱导的TC和CE升高。番红花可以抑制Ox-LDL诱导的SMC增殖。在存在或不存在细胞外Ca（2+）的情况下，番红花浓度依赖性抑制120mg.L（-）（1）Ox-LDL诱导的[Ca（2 +）]（i）升高，在不存在细胞外Ca（2+），番红花可以以浓度依赖的方式抑制CHCl（3）诱导的[Ca（2 +）]（i）升高。结果表明，番红花可以抑制鹌鹑的动脉粥样硬化形成。番红花对内皮细胞有保护作用。番红花可以降低巨噬细胞中的CE，并吸收Ox-LDL，抑制泡沫细胞的形成，从而促进动脉粥样硬化的发生和发展。 Crocin可以抑制平滑肌细胞中[Ca（2 +）]（i）的升高，Ca（2+）是重要的第二信使，它调节着各种细胞过程，包括平滑肌细胞的增殖和基因表达。番红花通过降低在动脉粥样硬化的发生和发展中起重要作用的Ox-LDL的水平发挥抗动脉粥样硬化作用。

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