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Prenatal stress on the kinetic properties of Ca and K channels in offspringhippocampal CA3 pyramidal neurons

机译：产前应激对后海马CA3锥体神经元Ca和K通道动力学特性的影响

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Prenatal stress is known to cause neuronal loss and oxidative damage in the hippocampus of offspring rats. To further understand the mechanisms, the present study was undertaken to investigate the effects of prenatal stress on the kinetic properties of high-voltage-activated (HVA) Ca 4 and K+ channels in freshly isolated hippocampal CA3 pyramidal neurons of offspring rats. Pregnant rats in the prenatal stress group were exposed to restraint stress on days 14-20 of pregnancy three times daily for 45 min. The patch clamp technique was employed to record HVA Ca2+ and K+ channel currents. Prenatal stress significantly increased HVA Ca2+ channel disturbance including the maximal average HVA calcium peak current amplitude (-576.52+-7.03 pA in control group and -702.05+-6.82 pA in prenatal stress group, p<0.01), the maximal average HVA Ca2+ current density (-40.89+-0.31 pA/pF in control group and -49.44+-0.37 pA/pF in prenatal stress group, p<0.01), and the maximal average integral current of the HVA Ca2+ channel (106.81+-4.20 nA ms in control group and 133.49+-4.59 nA ms in prenatal stress group, p<0.Ql). The current-voltage relationship and conductance-voltage relationship of HVA Ca2+ channels and potassium channels in offspring CA3 neurons were not affected by prenatal stress. These data suggest that exposure of animals to stressful experience during pregnancy can exert effects on calcium ion channels of offspring hippocampal neurons and that the calcium channel disturbance may play a role in prenatal stress-induced neuronal loss and oxidative damage in offspring brain. 500Prenatal stress; Patch clamp; CA3 neurons; Hippocampus; Ca2H channels
机译：众所周知，产前压力会在后代大鼠的海马中引起神经元丢失和氧化损伤。为了进一步了解其机制，本研究旨在研究产前应激对新生大鼠海马CA3锥体神经元新鲜分离的高压激活（HVA）Ca 4和K +通道动力学特性的影响。产前应激组的妊娠大鼠在妊娠第14-20天每天3次暴露于束缚压力下，持续45分钟。采用膜片钳技术记录HVA Ca2 +和K +通道电流。产前应激显着增加了HVA Ca2 +通道干扰，包括最大平均HVA钙峰值电流幅度（对照组为-576.52 + -7.03 pA，产前应激组为-702.05 + -6.82 pA，p <0.01），最大平均HVA Ca2 +电流密度（对照组为-40.89 + -0.31 pA / pF，产前应激组为-49.44 + -0.37 pA / pF，p <0.01），以及HVA Ca2 +通道的最大平均积分电流（106.81 + -4.20 nA ms对照组和产前应激组为133.49 + -4.59 nA ms，p <0.Q1）。产后应激不会影响后代CA3神经元HVA Ca2 +通道和钾通道的电流-电压关系和电导-电压关系。这些数据表明，动物在怀孕期间承受压力经历可以对后代海马神经元的钙离子通道产生影响，并且钙通道紊乱可能在出生后应激诱导的后代神经元损失和氧化损伤中起作用。 500产前压力;贴片夹; CA3神经元;海马; Ca2H通道

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