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首页> 外文期刊>Life sciences >Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage
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Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage

机译：环巴胺处理人胚胎干细胞，然后在人星形胶质细胞培养基中培养，促进分化为表达巢蛋白和GFAP的星形细胞谱系

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摘要

Human embryonic stem cells (hESCs) are able to differentiate into various cell types, including neuronal cells and glial cells. However, little information is available regarding astrocyte differentiation. This report describes the differentiation of hESCs into nestin- and GFAP-expressing astrocytes following treatment with cyclopamine, which is an inhibitor of Hedgehog (Hh) signaling, and culturing in human astrocyte medium (HAM). In hESCs, cyclopamine treatment suppressed the expression of Hh signaling molecules, the Hh signaling target gene, and ESC-specific markers. Clyclopamine also induced the differentiation of the cells at the edges of the hESC colonies, and these cells stained positively for the early neural marker nestin. Subsequent culturing in HAM promoted the expression of the astrocyte-specific marker GFAP, and these cells were also nestin-positive. These findings indicate that treatment with cyclopamine followed by culturing in HAM leads to the differentiation of hESCs into nestin- and GFAP-expressing astrocytic lineage. (c) 2006 Elsevier Inc. All rights reserved.
机译：人类胚胎干细胞（hESCs）能够分化为多种细胞类型，包括神经元细胞和神经胶质细胞。但是，关于星形胶质细胞分化的信息很少。该报告描述了在用环巴胺（hedgehog（Hh）信号的抑制剂）处理并在人星形胶质细胞培养基（HAM）中培养后，将hESCs分化为表达巢蛋白和GFAP的星形胶质细胞。在hESC中，环巴胺处理可抑制Hh信号分子，Hh信号靶基因和ESC特异性标记的表达。环丙胺还可以诱导hESC菌落边缘细胞的分化，这些细胞对早期神经标志物Nestin的染色呈阳性。随后在HAM中的培养促进了星形胶质细胞特异性标记GFAP的表达，并且这些细胞也是Nestin阳性的。这些发现表明用环巴胺处理，然后在HAM中培养可导致hESCs分化为表达Nestin和GFAP的星形细胞。 （c）2006 Elsevier Inc.保留所有权利。

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