• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Rat organic solute carrier protein l (rOscpl) mediated the transport of organic solutes in Xenopus laevis oocytes: Isolation and pharmacological characterization of r0scpl
【24h】

Rat organic solute carrier protein l (rOscpl) mediated the transport of organic solutes in Xenopus laevis oocytes: Isolation and pharmacological characterization of r0scpl

机译：大鼠有机溶质载体蛋白l（rOscpl）介导非洲爪蟾卵母细胞中有机溶质的运输：r0scpl的分离和药理学表征

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rat organic solute carrier protein I (rOscp1) was isolated from a rat testis cDNA library. Isolated rOscp1 cDNA consisted of 1089 base pairs that encoded a 363-amino acid protein, and the amino acid sequence was 88% and 93% identical to that of human OSCP1 (hOSCP1) and mouse Oscp1 (mOscp1), respectively. The message for rOscp1 is highly detected in rat testis. When expressed in X oocytes, rOscp1 mediated the high affinity transport of p-aminohippurate (PAH) with a K value of 15.7 +/- 1.9 mu M, and rOscp1-mediated organic solutes were exhibited in time- and Na+-independent manners. rOscp1 also transported various structurally heterogenous compounds such as testosterone, dehydroepiandrosterone sulfate (DHEA-S), and taurocholate with some differences in substrate specificity compared with hOSCP1. Immurrohistochemical analysis revealed that the rOscp1 protein is localized in the basal membrane side of Sertoli cells as observed in mouse testis [Kobayashi et al., 2007; Kobayashi, Y., Tsuchiya, A., Hayashi, T., Kohyama, N., Ohbayashi, M., Yamamoto, T., 2007. Isolation and characterization of polyspecific mouse organic solute carrier protein I (mOscp1). Drug Metabolism and Disposition 35 (7), 1239-12451]. Thus, the present results indicate that a newly isolated cDNA clone, rOscp1, is a polyspecific organic solute carrier protein with some differences in substrate specificity compared with human and mouse OSCP1. (C) 2007 Elsevier Inc. All rights reserved.
机译：从大鼠睾丸cDNA文库中分离出大鼠有机溶质载体蛋白I（rOscp1）。分离的rOscp1 cDNA由1089个碱基对组成，编码363个氨基酸的蛋白质，其氨基酸序列分别与人OSCP1（hOSCP1）和小鼠Oscp1（mOscp1）相同，分别为88％和93％。在大鼠睾丸中高度检测到rOscp1的消息。当在X卵母细胞中表达时，rOscp1介导对氨基氨基嘌呤（PAH）的高亲和力转运，K值为15.7 +/- 1.9μM，并且rOscp1介导的有机溶质以时间和Na +独立的方式展现。 rOscp1还转运了各种结构异质的化合物，例如睾丸激素，硫酸脱氢表雄酮（DHEA-S）和牛磺胆酸盐，与hOSCP1相比底物特异性有所不同。免疫组织化学分析表明，rOscp1蛋白位于小鼠睾丸中，位于Sertoli细胞的基底膜侧[Kobayashi等，2007; Nordon等。 Y. Kobayashi，A. Tsuchiya，A.，Hayashi，T.，Kohyama，N.，Ohbayashi，M.，Yamamoto，T.，2007。多特异性小鼠有机溶质载体蛋白I（mOscp1）的分离和表征。药物代谢与处置35（7），1239-12451]。因此，本结果表明，新分离的cDNA克隆rOscp1是一种多特异性有机溶质载体蛋白，与人和小鼠的OSCP1相比，底物特异性有所不同。 （C）2007 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号