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首页> 外文期刊>Cell and Tissue Research >Diffraction-unlimited imaging: from pretty pictures to hard numbers
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Diffraction-unlimited imaging: from pretty pictures to hard numbers

机译：衍射无限成像：从漂亮的图片到硬数字

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摘要

Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative insights from these techniques. In this work, we review the recent developments on sub-diffraction quantization that have arisen for the various techniques currently in use. We pay particular attention to the information that can be obtained but also the practical problems that can be faced, and provide suggestions for solutions or workarounds. We also show that these quantitative metrics not only provide a way to turn raw data into hard statistics but also help to understand the features and pitfalls associated with sub-diffraction imaging. Ultimately, these developments will lead to a highly standardized and easily applicable toolbox of techniques, which will find widespread application in the scientific community.
机译：衍射无限制的荧光成像技术可以以前所未有的细节水平观察完整的，高度异构的系统。除了获取详细的图片外，现在越来越多的工作集中在从这些技术中获得定量的见解。在这项工作中，我们回顾了当前使用的各种技术在亚衍射量化方面的最新发展。我们特别关注可以获得的信息以及可能面临的实际问题，并为解决方案或解决方法提供建议。我们还表明，这些定量指标不仅提供了一种将原始数据转化为硬统计量的方法，而且还有助于理解与亚衍射成像相关的特征和陷阱。最终，这些发展将导致高度标准化且易于应用的技术工具箱，这些工具箱将在科学界得到广泛应用。

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