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首页> 外文期刊>Life sciences >Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage
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Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage

机译：曲安奈德可保护大鼠视网膜免受STZ诱导的急性炎症和早期血管渗漏

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摘要

Streptozotocin (STZ) has been commonly used to induce in vivo and in vitro hyperglycemic diabetes and its toxicity leads to inflammation and vascular injury. Triamcinolone acetonide (TA), as an anti-angiogenic/anti-inflammatory drug, is clinically used to improve the visual acuity in neovascular and edematous ocular diseases. The aim of this study was to investigate the effect of TA on early inflammation and vascular leakage in the retina of STZ-induced hyperglycemic rats. Hyperglycemia was induced in 8-week-old male Sprague-Dawley (SD) rats by a single intraperitoneal injection of STZ (65 mg/kg); only rats with blood glucose levels > 13.9 mmol/l 1 day after STZ injection were included in STZ-hyperglycemic group. Sex- and age-matched SD rats injected with buffer were used as the control group. One day before STZ and buffer injection, 2 mu l TA (4 mg/ml in saline) and 2 mu l saline were intravitreal-injected into the right and the left eyes of rats, respectively. Retinal vascular leakage was measured using the Evans-blue method. Changes in pro-inflammatory target genes, such as tumor necrotic factor (TNF)-alpha, intracellular adhesion molecule (ICAM)-1, and vascular endothelial growth factor (VEGF) were assessed by immunoblottings, immunostaining, and ELISA analyses. Vascular hyperleakage and up-regulation of most pro-inflammatory genes peaked within a few days after STZ injection and had recovered. However, these changes were blocked by TA pretreatment. Our data suggest that TA controls STZ-induced early vascular leakage and temporary pro-inflammatory signals in the rat retina. (C) 2007 Elsevier Inc. All rights reserved.
机译：链脲佐菌素（STZ）已普遍用于诱导体内和体外高血糖糖尿病，其毒性导致炎症和血管损伤。曲安奈德（TA），作为一种抗血管生成/消炎药，在临床上用于改善新血管和水肿性眼病的视敏度。这项研究的目的是调查TA对STZ诱导的高血糖大鼠视网膜早期炎症和血管渗漏的影响。通过单次腹膜内注射STZ（65 mg / kg）在8周大的雄性Sprague-Dawley（SD）大鼠中诱发高血糖。 STZ-高血糖组仅包括在STZ注射后1天血糖水平> 13.9 mmol / l的大鼠。性别和年龄相匹配的SD大鼠注射缓冲液作为对照组。在STZ和缓冲液注射前一天，分别向大鼠的右眼和左眼玻璃体内注射2μlTA（4 mg / ml生理盐水）和2μl生理盐水。视网膜血管渗漏使用伊文思蓝法测量。通过免疫印迹，免疫染色和ELISA分析评估促炎靶基因的变化，例如肿瘤坏死因子（TNF）-α，细胞内粘附分子（ICAM）-1和血管内皮生长因子（VEGF）。注射STZ后几天内，大多数促炎基因的血管过度渗漏和上调达到峰值，并且已经恢复。但是，这些变化被TA预处理阻止了。我们的数据表明TA可控制STZ诱导的大鼠视网膜早期血管渗漏和暂时的促炎信号。 （C）2007 Elsevier Inc.保留所有权利。

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