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首页> 外文期刊>Life sciences >Protective effect of cyclosporin A on brain injury in rats with acute necrotic pancreatitis.
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Protective effect of cyclosporin A on brain injury in rats with acute necrotic pancreatitis.

机译：环孢菌素A对急性坏死性胰腺炎大鼠脑损伤的保护作用。

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AIMS: This study was designed to evaluate the protective effect of cyclosporin A (CsA) on brain injury in rats with acute necrotic pancreatitis (ANP) in order to provide a scientific basis for the use of the drug in treating brain injury caused by pancreatitis. MAIN METHODS: The rats were divided into a sham group, an ANP group and an ANP+CsA group. The ANP model was induced by administering 5% sodium taurocholate through the biliopancreatic duct. Five minutes before the preparation of the ANP model, 1 ml of CsA (10mg/kg) was injected in a clinically used pharmaceutical formulation (Sandimmun) via the dorsal vein of the penis. Twelve hours after the model was established, samples were taken from the rats in all groups for measurement of appropriate indexes. The serum levels of pro-inflammatory tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and anti-inflammatory interleukin 10 (IL-10) were determined by ELISA. The pancreatic mRNA expressions of these cytokines were evaluated by RT-PCR. Brain water content was tested by the dry-wet method, and brain malondialdehyde (MDA) content was detected by the chemical colorimetry method. KEY FINDINGS: Both the serum levels and the pancreatic tissue mRNA expression of TNF-alpha and IL-1beta, as well as the brain water content and brain MDA content, were significantly increased in the ANP group. CsA treatment noticeably reduced both the serum levels and the pancreatic mRNA expression of TNF-alpha and IL-1beta and decreased brain water and MDA contents. In contrast to the pro-inflammatory cytokines, the serum levels and the pancreatic tissue mRNA expression of IL-10 were markedly increased by the injection of CsA. SIGNIFICANCE: CsA could alleviate acute pancreatitis-associated brain injury.
机译：目的：本研究旨在评估环孢菌素A（CsA）对急性坏死性胰腺炎（ANP）大鼠脑损伤的保护作用，从而为该药物用于治疗胰腺炎引起的脑损伤提供科学依据。主要方法：将大鼠分为假手术组，ANP组和ANP + CsA组。通过经由胆胰管施用5％牛磺胆酸钠来诱导ANP模型。制备ANP模型前五分钟，通过阴茎背静脉将1 ml CsA（10mg / kg）注射到临床使用的药物制剂（Sandimmun）中。建立模型后十二小时，从所有组的大鼠中取样以测量适当的指标。通过ELISA测定血清促炎性肿瘤坏死因子-α（TNF-α），白介素1β（IL-1β）和抗炎性白介素10（IL-10）的水平。通过RT-PCR评估这些细胞因子的胰腺mRNA表达。用干湿法检测脑水含量，用化学比色法检测脑丙二醛（MDA）含量。主要发现：ANP组血清TNF-α和IL-1β的水平和胰腺组织mRNA表达，以及脑水含量和脑MDA含量均显着增加。 CsA处理显着降低了TNF-α和IL-1beta的血清水平和胰腺mRNA表达，并降低了脑水和MDA含量。与促炎细胞因子相反，通过注射CsA，IL-10的血清水平和胰腺组织mRNA表达显着增加。意义：CsA可以减轻急性胰腺炎相关的脑损伤。

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