• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >High-throughput screening identifies CHMP4A associated with hypoxia-inducible factor 1.
【24h】

High-throughput screening identifies CHMP4A associated with hypoxia-inducible factor 1.

机译：高通量筛选可识别与缺氧诱导因子1相关的CHMP4A。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIMS: Tumor hypoxia is a common phenomenon and hypoxia-inducible factor-1 is the transcription factor that is most closely associated with hypoxia. Hypoxia-inducible factor-1 is overexpressed in most solid tumors and plays a vital role in hypoxic acclimatization, energy metabolism, tumor angiogenesis, tumor invasion, and drug tolerance in cancer cells. We aimed to identify novel human genes associated with the stability and transcriptional activity of hypoxia-inducible factor-1. MAIN METHODS: A cell-based dual luciferase reporter system based on a hypoxia responsive element luciferase reporter gene was constructed to screen 409 novel human genes cloned in our lab. Western blot analysis was used to examine the changes in the expression level of hypoxia-inducible factor-1 alpha, and RT-PCR analysis was used to detect the transcription level of adenylate kinase 3. KEY FINDINGS: Our results demonstrated that chromatin-modifying protein 4A could significantly up-regulate the hypoxia responsive element luciferase activity under both normoxic and cobalt chloride-induced hypoxic environment in HeLa cells. Moreover, Chromatin-modifying protein 4A could increase the expression of hypoxia-inducible factor-1 alpha protein under normoxic condition, and enhance the transcription level of adenylate kinase 3, which is one of the target genes of hypoxia-inducible factor-1. SIGNIFICANCE: The functional screening platform therefore can be applied for the high-throughput screening of hypoxia-inducible factor-1-related genes, which would provide new insights into underlying molecular mechanisms that may regulate hypoxia in mammalian cells.
机译：目的：肿瘤缺氧是一种常见现象，缺氧诱导因子-1是与缺氧最密切相关的转录因子。缺氧诱导因子-1在大多数实体瘤中过表达，并且在低氧适应，能量代谢，肿瘤血管生成，肿瘤侵袭和癌细胞的耐药性中起着至关重要的作用。我们旨在鉴定与缺氧诱导因子-1的稳定性和转录活性相关的新型人类基因。主要方法：构建基于缺氧反应元件荧光素酶报告基因的基于细胞的双重荧光素酶报告系统，以筛选在我们实验室中克隆的409个新人类基因。 Western印迹分析用于检测缺氧诱导因子-1α表达水平的变化，RT-PCR分析用于检测腺苷酸激酶3的转录水平。关键发现：我们的结果证明了染色质修饰蛋白在常氧和氯化钴诱导的低氧环境下，图4A可以显着上调缺氧反应元件荧光素酶的活性。此外，染色质修饰蛋白4A可在常氧条件下增加缺氧诱导因子-1α蛋白的表达，并增强腺苷酸激酶3的转录水平，这是缺氧诱导因子-1的靶基因之一。意义：因此，该功能筛选平台可用于高通量筛选缺氧诱导因子-1相关基因，这将为调节哺乳动物细胞缺氧的潜在分子机制提供新见解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号