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首页> 外文期刊>Life sciences >Regulation of histidase gene expression by glucagon, hydrocortisone and protein-free/high carbohydrate diet in the rat.
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Regulation of histidase gene expression by glucagon, hydrocortisone and protein-free/high carbohydrate diet in the rat.

机译：胰高血糖素，氢化可的松和无蛋白/高碳水化合物饮食在大鼠中对组氨酸酶基因表达的调节。

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摘要

The effect of glucagon and hydrocortisone was investigated to understand the mechanism of induction of hepatic histidase gene. In this study, glucagon (0.6 mg/100 g body wt/d) was injected to rats fed 10% casein diet. After 3 h of the last injection, histidase activity and amount of enzyme were induced by 3 fold and histidase mRNA concentration by 6 fold. Injection of hydrocortisone (2 mg/100 g body wt/d) increased 100% histidase activity and mRNA concentration and by 150% the amount of enzyme after 3 h of the last injection. These results indicate that glucagon is a better inductor of histidase gene expression than hydrocortisone. Another purpose of the study was to evaluate if a protein-free/high carbohydrate diet could reverse the induction of Hal expression produced by a high protein diet. Hal activity, amount of enzyme and mRNA concentration was repressed by 68, 88 and 95% respectively by a protein-free/high carbohydrate diet. Injection of glucagon reversed partially the effect of a high carbohydrate diet, however, injection of hydrocortisone under the concentration used in these experiments did not reverse the effect of a high carbohydrate diet. These results support the evidence that hepatic histidase gene expression is probably regulated transcriptionally by hormones.
机译：研究了胰高血糖素和氢化可的松的作用，以了解诱导肝组氨酸酶基因的机制。在这项研究中，将胰高血糖素（0.6 mg / 100 g体重/天）注射给饲喂10％酪蛋白饮食的大鼠。最后一次注射3小时后，组氨酸酶活性和酶量被诱导3倍，组氨酸酶mRNA浓度被诱导6倍。在最后一次注射后3小时，注射氢化可的松（2 mg / 100 g体重/ d）可增加100％的组氨酸酶活性和mRNA浓度，并使酶量增加150％。这些结果表明胰高血糖素比氢化可的松更好地诱导组氨酸酶基因表达。该研究的另一个目的是评估无蛋白/高碳水化合物饮食是否可以逆转由高蛋白饮食产生的Hal表达的诱导。无蛋白/高碳水化合物饮食分别使Hal活性，酶量和mRNA浓度分别降低68％，88％和95％。胰高血糖素的注射部分地逆转了高碳水化合物饮食的作用，但是，在这些实验中使用的浓度下注射氢化可的松并没有逆转高碳水化合物饮食的作用。这些结果支持了肝组氨酸酶基因表达可能受激素转录调控的证据。

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