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首页> 外文期刊>Life sciences >Possible autocrine enkephalin regulation of catecholamine release in human pheochromocytoma cells.
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Possible autocrine enkephalin regulation of catecholamine release in human pheochromocytoma cells.

机译：人嗜铬细胞瘤细胞中儿茶酚胺释放的可能自分泌脑啡肽调节。

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AIMS: Pheochromocytomas are catecholamine-secreting tumors that also synthesize and secrete several neuropeptides, including opioids. A negative regulation of catecholamine secretion by opioids has been postulated in chromaffin cells. However, results obtained so far are contradictory when referred to human pheochromocytomas. The aim of this study was to define the role of locally produced enkephalins on catecholamine release in human pheochromocytoma cells. MAIN METHODS: Cells obtained from eleven human pheochromocytomas of different genetic origins were cultured for 5 days. Cultures were maintained under basal condition or under enkephalin, dexamethasone and naloxone alone or in combination with enkephalin or dexamethasone-stimulated conditions. Catecholamine and enkephalin levels in the culture medium were measured by HPLC-ED and RIA respectively. KEY FINDINGS: Enkephalin induced a decrease in norepinephrine levels in all tumor cultures. Dexamethasone treatment, which increased enkephalin levels, also decreased catecholamine levels. On the other hand, the addition of naloxone to the cultures reverted to normal the inhibitory action exerted by enkephalin and dexamethasone treatments. SIGNIFICANCE: These results suggest the existence of an autocrine negative regulatory loop exerted by enkephalin on norepinephrine release in human pheochromocytoma cells.
机译：目的：嗜铬细胞瘤是分泌儿茶酚胺的肿瘤，还合成和分泌多种神经肽，包括阿片类药物。阿片类药物对儿茶酚胺分泌的负调节已被假定在嗜铬细胞中。但是，迄今为止所获得的结果在涉及人类嗜铬细胞瘤时是矛盾的。这项研究的目的是确定局部产生的脑啡肽对人嗜铬细胞瘤细胞中儿茶酚胺释放的作用。主要方法：从11种不同遗传来源的人类嗜铬细胞瘤获得的细胞培养5天。将培养物维持在基础条件下或单独在脑啡肽，地塞米松和纳洛酮下或与脑啡肽或地塞米松刺激的条件下组合。分别通过HPLC-ED和RIA测量培养基中的儿茶酚胺和脑啡肽水平。主要发现：脑啡肽诱导所有肿瘤培养物中去甲肾上腺素水平降低。地塞米松治疗增加了脑啡肽水平，也降低了儿茶酚胺水平。另一方面，向培养物中添加纳洛酮使脑啡肽和地塞米松治疗产生的抑制作用恢复正常。意义：这些结果表明脑啡肽在人嗜铬细胞瘤细胞中释放去甲肾上腺素时，存在自分泌负调控环。

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