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首页> 外文期刊>Life sciences >Neural progenitor cells derived from adult bone marrow mesenchymal stem cells promote neuronal regeneration
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Neural progenitor cells derived from adult bone marrow mesenchymal stem cells promote neuronal regeneration

机译：来自成年骨髓间充质干细胞的神经祖细胞促进神经元再生

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Aim: It is well known that neural stem/progenitor cells (NS/PC) are an ideal cell type for the treatment of central nervous system (CNS) disease. However, ethical problems have severely hampered fetal NS/PC from being widely used as a source for stem cell therapy. Recently, it has been demonstrated that autologous bone marrow mesenchymal stem cells (BMSC) can transdifferentiate into neural progenitor cells (NPC). The biological function of BMSC derived NPC (MDNPC) in neuronal systems remains unknown. In the present study, we aimed to investigate whether MDNPC can promote in vitro neural regeneration, a process comprising mainly the generation of neurons and neurotransmitters. Main methods: We co-cultured BMSC, MDNPC or fetal NS/PC with PC12 cells and studied their roles on proliferation, neurite formation and dopamine release from PC12 cells. Furthermore, we also explored the mechanisms by which MDNPC regulate dopamine secretion from PC12 derived neural cells using Western blot. Key findings: We found that both MDNPC and NS/PC had similar morphologies and there were no significant differences between MDNPC and NS/PC in promoting PC12 cell proliferation, neurite outgrowth, and dopamine release. We also demonstrated that NS/PC induced dopamine secretion was associated with an upregulation of dopamine transporter (DAT) levels. Significance: In summary, MDNPC were comparable to NS/PC in promoting neural regeneration, indicating that MDNPC are a promising candidate source of neural stem cells for the treatment of neurological diseases.
机译：目的：众所周知，神经干/祖细胞（NS / PC）是治疗中枢神经系统（CNS）疾病的理想细胞类型。但是，伦理问题严重阻碍了胎儿NS / PC被广泛用作干细胞治疗的来源。最近，已经证明自体骨髓间充质干细胞（BMSC）可以转分化为神经祖细胞（NPC）。 BMSC衍生的NPC（MDNPC）在神经元系统中的生物学功能仍然未知。在本研究中，我们旨在研究MDNPC是否可以促进体外神经再生，该过程主要包括神经元和神经递质的产生。主要方法：我们将BMSC，MDNPC或胎儿NS / PC与PC12细胞共培养，并研究它们在PC12细胞增殖，神经突形成和多巴胺释放中的作用。此外，我们还使用Western印迹探索了MDNPC调节PC12衍生神经细胞多巴胺分泌的机制。关键发现：我们发现MDNPC和NS / PC的形态相似，并且MDNPC和NS / PC在促进PC12细胞增殖，神经突生长和多巴胺释放方面没有显着差异。我们还证明了NS / PC诱导的多巴胺分泌与多巴胺转运蛋白（DAT）水平的上调有关。意义：总之，MDNPC在促进神经再生方面可与NS / PC媲美，表明MDNPC是治疗神经疾病的神经干细胞的有希望的候选来源。

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