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首页> 外文期刊>Life sciences >Ovarian steroids reduced apoptotic death in SV40 temperature-sensitive mutant virus transformed uterine epithelial cells.
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Ovarian steroids reduced apoptotic death in SV40 temperature-sensitive mutant virus transformed uterine epithelial cells.

机译：卵巢类固醇可降低SV40温度敏感突变病毒转化的子宫上皮细胞的凋亡死亡。

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摘要

Ovarian steroids have been shown to inhibit uterine cell death in vivo. In this study, we investigated whether ovarian steroids regulated cell death in an uterine epithelial cell line transformed with SV40 temperature-sensitive (ts) mutant virus. To assess cell death rate, cells were grown at permissive temperature (34 degrees C) and pulsed with 3H-thymidine. The retention of incorporated radioactivity was then examined after a temperature shift to nonpermissive temperature (40 degrees C) in the absence or presence of estradiol and progesterone. When cells were continuously cultured at 34 degrees C, cell number increased rapidly and most of radioactivity was retained in the attached cells. However, the temperature shift from 34 degrees C to 40 degrees C resulted in a decrease in cell number and radioactivity in attached cells. Estradiol and progesterone attenuated this temperature shift-induced cell death. Morphological examination with Hoechst 33258 staining revealed that the temperature shift increased the percentage of apoptotic death. The treatment of ovarian steroids reduced the extent of apoptotic death. Our studies demonstrated that ovarian steroids could act directly on uterine epithelial cells to reduce apoptotic death in culture.
机译：卵巢类固醇已被证明在体内抑制子宫细胞死亡。在这项研究中，我们调查了卵巢类固醇是否调节了用SV40温度敏感（ts）突变病毒转化的子宫上皮细胞系中的细胞死亡。为了评估细胞死亡率，使细胞在允许的温度（34摄氏度）下生长并用3H-胸苷脉冲处理。然后在不存在或存在雌二醇和孕酮的情况下，将温度转变为非许可温度（40摄氏度）后，检查结合放射性的保留。当在34℃下连续培养细胞时，细胞数迅速增加，大部分放射性保留在附着的细胞中。但是，从34摄氏度到40摄氏度的温度变化导致细胞数量减少，并降低了附着细胞的放射性。雌二醇和孕酮减弱了这种温度变化诱导的细胞死亡。 Hoechst 33258染色的形态学检查表明，温度变化增加了细胞死亡的百分比。卵巢类固醇的治疗减少了细胞凋亡的程度。我们的研究表明，卵巢类固醇可以直接作用于子宫上皮细胞，从而减少培养物中的细胞凋亡死亡。

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