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首页> 外文期刊>Life sciences >Type 2 corticotropin-releasing hormone receptor mRNA expression in the heart in hypertensive rats.
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Type 2 corticotropin-releasing hormone receptor mRNA expression in the heart in hypertensive rats.

机译：高血压大鼠心脏中2型促肾上腺皮质激素释放激素受体mRNA的表达。

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摘要

Novel subtype of corticotropin-releasing hormone receptor (CRHR), designated type-2 CRHR (CRHR-2), mRNA was expressed not only in the central nervous system but also in the peripheral tissues such as the heart and skeletal muscle. The previous finding that type-1 CRHR mRNA is not detected in heart leads us to speculate that systemic administration of CRH induces hypotensive effects through CRHR-2, and that alterations in CRHR-2 in the heart may be implicated in blood pressure regulation. Therefore we examined CRHR-2 mRNA expression in the heart (at the level of ventricle) in spontaneously hypertensive rats (SHR) or DOCA-salt hypertensive rats (DOCA) using in situ hybridization histochemistry, compared to age-matched normotensive control rats. CRHR-2 mRNA levels in the heart were significantly higher in 7-week-old SHR than in 12-week-old SHR. Furthermore, CRHR-2 mRNA levels in SHR heart were significantly higher than those in normotensive controls, Wistar-Kyoto rats (WKY), at both 7 and 12 weeks of age. In contrast, CRHR-2 mRNA levels in DOCA heart were significantly lower than that of sham-operated controls after 6-weeks of treatment. Thus, alterations of CRHR-2 mRNA are dependent on the strain or experimental condition rather than as a consequence of hypertension. Plasma CRH levels in SHR or DOCA were not different from their normotensive control rats. CRH content in the ventricular heart of SHR or DOCA were also similar to normotensive controls. These results suggest that heart CRHR-2 mRNA levels are not influenced by circulating or locally existing CRH. Since alterations in heart CRHR-2 mRNA, as seen in SHR and DOCA, were bi-directional, the role of heart CRHR-2 in the regulation of hypertension remains to be elucidated.
机译：促肾上腺皮质激素释放激素受体（CRHR）的新亚型，称为2型CRHR（CRHR-2），mRNA不仅在中枢神经系统中表达，而且在诸如心脏和骨骼肌等周围组织中表达。先前在心脏中未检测到1型CRHR mRNA的发现使我们推测全身施用CRH可通过CRHR-2引起降压作用，而心脏CRHR-2的改变可能与血压调节有关。因此，与年龄匹配的血压正常对照组相比，我们使用原位杂交组织化学方法检查了自发性高血压大鼠（SHR）或DOCA盐高血压大鼠（DOCA）心脏中CRHR-2 mRNA的表达（在心室水平）。在7周龄SHR中，心脏的CRHR-2 mRNA水平明显高于在12周龄SHR中。此外，在7周和12周龄时，SHR心脏的CRHR-2 mRNA水平均显着高于血压正常对照Wistar-Kyoto大鼠（WKY）。相比之下，治疗6周后，DOCA心脏中的CRHR-2 mRNA水平显着低于假手术对照组。因此，CRHR-2 mRNA的改变取决于菌株或实验条件，而不是高血压的结果。 SHR或DOCA中的血浆CRH水平与血压正常的大鼠没有差异。 SHR或DOCA的心室心脏中的CRH含量也与血压正常对照相似。这些结果表明，心脏CRHR-2 mRNA水平不受循环或局部存在的CRH的影响。如SHR和DOCA中所见，由于心脏CRHR-2 mRNA的改变是双向的，因此有待阐明心脏CRHR-2在调节高血压中的作用。

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