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首页> 外文期刊>Cell biology international. >Akebia saponin D, a saponin component from Dipsacus asper Wall, protects PC 12 cells against amyloid-beta induced cytotoxicity.
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Akebia saponin D, a saponin component from Dipsacus asper Wall, protects PC 12 cells against amyloid-beta induced cytotoxicity.

机译：七叶树皂苷中的皂苷成分Akebia皂苷D保护PC 12细胞免受β淀粉样蛋白诱导的细胞毒性。

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摘要

According to Traditional Chinese Medicine, Alzheimer's disease (AD) is regarded as senile dementia, and the etiopathogenesis lies in kidney deficiency during aging. Dipsacus asper Wall (DAW), a well-known traditional Chinese medicine for enhancing kidney activity, may possess the therapeutic effects against AD. Our objectives were to investigate the protective effects of DAW against the amyloid-beta peptide (A beta)-induced cytotoxicity and explore its major active components. Injury of PC 12 cells mediated by A beta(25-35) was adopted to assess the cytoprotective effects of DAW aqueous extract and various fractions. Salvianolic acid B, a polyphenol compound isolated from Salvia miltiorrhiza, was employed as a positive control agent due to its markedly protective effect against neurotoxicity of amyloid beta. Five chemical fractions (i.e. alkaloids, essential oil, saponins, iridoid glucoside and polysaccharides) were prepared for activity test and analyzed by HPLC for active components identification. In addition, Akebia saponin D (the most important compound in DAW saponins) and hederagenin (the mother nucleus of akebia saponin D) were prepared for testing of their activity. DAW water extract, saponins fraction and akebia saponin D had the neuroprotective capacity to antagonize A beta(25-35)-induced cytotoxicity in PC 12 cells. In contrast, other fractions and hederagenin had no cytoprotective action. This research suggests that DAW may represent a potential treatment strategy for AD and akebia saponin D is one of its active components.
机译：根据中医药，阿尔茨海默氏病（AD）被认为是老年性痴呆，其病因在于衰老过程中的肾虚。 Dipsacus asper Wall（DAW）是一种众所周知的增强肾脏活性的中药，可能具有抗AD的治疗作用。我们的目标是研究DAW对淀粉样β肽（A beta）诱导的细胞毒性的保护作用，并探讨其主要活性成分。通过Aβ（25-35）介导的PC 12细胞的损伤来评估DAW水提取物和各种组分的细胞保护作用。从丹参中分离出的多酚化合物丹酚酸B由于对淀粉样β的神经毒性具有明显的保护作用，因此被用作阳性对照。制备了五个化学级分（即生物碱，香精油，皂苷，虹彩糖苷和多糖）进行活性测试，并通过HPLC分析以鉴定活性成分。此外，还准备了Akebia皂苷D（DAW皂苷中最重要的化合物）和hederagenin（akebia皂苷D的母核）来测试其活性。 DAW水提取物，皂苷组分和苏木皂苷D具有拮抗PC 12细胞中Abeta（25-35）诱导的细胞毒性的神经保护能力。相比之下，其他级分和乙酰二十碳皂苷没有细胞保护作用。这项研究表明，DAW可能代表了AD的潜在治疗策略，而苏木皂甙D是其有效成分之一。

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