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首页> 外文期刊>Cell biology international. >Bcl-2 is an integral component of mitotic chromosomes.
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Bcl-2 is an integral component of mitotic chromosomes.

机译：Bcl-2是有丝分裂染色体的组成部分。

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We previously demonstrated that phospho-Thr56 Bcl-2 colocalizes with Ki-67 and nucleolin in nuclear structures in prophase cells and is detected on mitotic chromosomes in later mitotic phases. To gain insight into the fine localization of Bcl-2 on mitotic chromosomes, we further investigated Bcl-2 localization by immunostaining of Bcl-2 with known components of metaphase chromosomes and electron microscopic immunocytochemistry. Immunofluorescence analysis on HeLa mitotic cells together with chromatin immunoprecipitation assays showed that Bcl-2 is associated with the condensed chromatin. Co-immunostaining experiments performed on mitotic chromosome spreads demonstrated that Bcl-2 is not localized on the longitudinal axis of chromatids with the condensin complex, but partially colocalizes with histone H3 on some regions of the mitotic chromosome. Finally, most of the Bcl-2 staining overlaps with Ki-67 staining at the chromosome periphery. Bcl-2 localization at the periphery and over the mitotic chromosome was confirmed by immunoelectron microscopy on mitotic cells. Our results indicate that Bcl-2 is an integral component of the mitotic chromosome.
机译：我们以前证明磷酸化-Thr56 Bcl-2与前体细胞核结构中的Ki-67和核仁素共定位，并在以后的有丝分裂期的有丝分裂染色体上检测到。为了深入了解Bcl-2在有丝分裂染色体上的精细定位，我们通过用中期染色体的已知成分对Bcl-2进行免疫染色和电子显微镜免疫细胞化学进一步研究了Bcl-2的定位。 HeLa有丝分裂细胞的免疫荧光分析以及染色质免疫沉淀分析表明Bcl-2与浓缩的染色质相关。在有丝分裂染色体上进行的共免疫染色实验表明，Bcl-2并不与凝聚素复合物位于染色单体的纵轴上，而是与组蛋白H3在有丝分裂染色体的某些区域上部分共定位。最后，大多数Bcl-2染色在染色体外围与Ki-67染色重叠。通过在有丝分裂细胞上的免疫电子显微镜证实了Bcl-2定位在外周和有丝分裂染色体上。我们的结果表明Bcl-2是有丝分裂染色体的组成部分。

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