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首页> 外文期刊>Life sciences >Gender difference in functional properties of Na,K-ATPase in the heart of spontaneously hypertensive rats.
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Gender difference in functional properties of Na,K-ATPase in the heart of spontaneously hypertensive rats.

机译：自发性高血压大鼠心脏Na，K-ATPase功能特性的性别差异。

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摘要

The aim of present study was the investigation of functional properties of the cardiac Na,K-ATPase in 16 weeks old male and female spontaneously hypertensive rats (SHR). The Na,K-ATPase activity in the presence of increasing concentrations of ATP, as well as Na(+) was lower in SHR of both genders, as compared to respective normotensive controls. Evaluation of kinetic parameters revealed a significant decrease of the maximum velocity (V(max)) in males (30% for ATP-activation, 40% for Na(+)-activation), as well as in females (24% for ATP, 29% for Na(+)), indicating a hypertension-induced diminution of the number of active enzyme molecules in cardiac sarcolemma. Insignificant changes were observed in the value of Michaelis-Menten constant (K(m)) in both cases. The concentration of sodium that gives half-maximal reaction velocity (K(Na)), increased by 38% in male and by 70% in female SHR. This impairment in the affinity of the Na(+)-binding site together with decreased number of active Na,K-ATPase molecules are probably responsible for the deteriorated enzyme-function in hearts of SHR. Direct comparison of SHR of both genders showed, that the enzyme from female hearts seems to be adapted better to hypertension as documented by its increased activity as a consequence of improved ability to bind and utilize ATP, as suggested by 32% decrease of K(m) value in females. In addition, the enzyme from female hearts is able to increase its activity (by 41%) in the presence of increasing sodium concentration even in the range where the enzyme from male hearts is already saturated.
机译：本研究的目的是调查16周大的雄性和雌性自发性高血压大鼠（SHR）心脏Na，K-ATPase的功能特性。与相应的血压正常对照组相比，在存在增加的ATP浓度以及Na（+）的情况下，两种性别的SHR中的Na，K-ATPase活性均较低。动力学参数的评估显示，男性（ATP激活30％，Na（+）激活40％）的最大速度（V（max））以及女性（ATP的24％， Na（+））为29％，表明高血压引起的心肌肉瘤中活性酶分子数量减少。在两种情况下，Michaelis-Menten常数（K（m））的值均无明显变化。产生最大反应速度一半（K（Na））的钠浓度在男性SHR中增加38％，在女性SHR中增加70％。 Na（+）结合位点的亲和力下降与活性Na，K-ATPase分子数量减少可能是SHR心脏中酶功能下降的原因。两种性别的SHR的直接比较表明，女性心脏中的酶似乎更适合高血压，这是由于其结合和利用ATP的能力提高所致，其活性增加而证明的，K（m）降低了32％ ）对女性的价值。另外，在钠浓度增加的情况下，即使在来自男性心脏的酶已经饱和的范围内，来自女性心脏的酶也能够增加其活性（41％）。

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