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LACK OF AN EMF-INDUCED GENOTOXIC EFFECT IN THE AMES ASSAY

机译：在AMES试验中缺乏由EMF引起的遗传氧化效应

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A few epidemiological studies have linked exposure to electromagnetic fields (EMF) and the incidence of cancer. Since many carcinogens are mutagens in the Ames assay, the purpose of this study was to determine if exposure of four tester strains of Salmonella typhimurium (TA97a, TA98, TA100, and TA102) to EMF would increase their rate of mutation. Parallel plate electrodes and Helmholtz coils were used to create uniform field properties (300 V/in., 0.3 mT). Separate and combined alternating electric and magnetic fields effects were studied at a combined field frequency of 60, 600, and 6000 Hz at room temperature. These fields did not elevate the temperature of the culture plates above room temperature. Petri dishes containing each tester strain in top agar were exposed to an electric field (E), magnetic field (M), combined electric and magnetic field (EM), or no additional field above ambient conditions in the lab (control). Four plates containing each strain were exposed in each condition: two plates had the appropriate positive-control mutagen for each strain included in the top agar and two plates did not. Plates were exposed to either E, M, EM, or control conditions at room temperature for 48 hr. and then incubated an additional 24 hr. at 37 deg. C. The plates containing mutagen in the top agar showed an increased number of colonies consistent with mutagenesis. However, the rate of mutation in the S. typhimurium strains TA97a, TA98, TA100, and TA102 in either the presence or absence of mutagen was not affected by 48 hr. exposure at room temperature to E, M, or EM fields at 60, 600, or 6000 Hz. [References: 24]
机译：一些流行病学研究已将暴露于电磁场（EMF）与癌症的发生率联系起来。由于在Ames分析中许多致癌物是诱变剂，因此本研究的目的是确定四种鼠伤寒沙门氏菌（TA97a，TA98，TA100和TA102）测试菌株暴露于EMF是否会增加其突变率。平行板电极和亥姆霍兹线圈用于产生均匀的场特性（300 V / in。，0.3 mT）。在室温下，分别以60、600和6000 Hz的组合磁场频率研究了分离的和组合的交变电场和磁场效应。这些场没有将培养板的温度升高到室温以上。在实验室中，将含有每种测试菌株的顶培养皿中的培养皿置于电场（E），磁场（M），电场和磁场组合（EM）或没有高于环境条件的其他电场下（对照）。在每种条件下暴露含有每种菌株的四块平板：两块平板针对上琼脂中包含的每种菌株具有适当的阳性对照诱变剂，而两块平板则没有。将板在室温下暴露于E，M，EM或对照条件下48小时。然后再孵育24小时。在37度C.在顶部琼脂中含有诱变剂的平板显示出与诱变相一致的菌落数量增加。但是，在有或没有诱变剂的情况下，鼠伤寒沙门氏菌菌株TA97a，TA98，TA100和TA102的突变率不受48小时的影响。在室温下暴露于60、600或6000 Hz的E，M或EM场。 [参考：24]

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