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Effect of cisplatin on brush border membrane enzymes and anti-oxidant system of rat intestine

机译：顺铂对大鼠肠道刷状缘膜酶和抗氧化系统的影响

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Cisplatin (CP) is a widely used antineoplastic agent which exhibits gastrointestinal toxicity. The present work was done to study the effect of administration of CP on brush border membrane (BBM) enzymes and anti-oxidant system of rat intestine. Male Wistar rats were given a single intraperitoneal dose of CP (6 mg/kg body weight) and then sacrificed 1, 3, 5 and 7 days after this treatment. Control animals were given saline only. The administration of CP led to significant decline in the specific activities of BBM enzymes both in the mucosal homogenates and isolated membrane vesicles. Kinetic studies showed that the V-max of the enzymes was decreased in BBM vesicles from CP treated rats while the K-m remained unchanged. The activities of catalase, Cu-Zn superoxide dismutase, glucose 6-phosphate dehydrogenase and glutathione reductase decreased while the activities of glutathione S-transferase and thioredoxin reductase increased in CP treated animals compared to the control group. Lipid peroxidation and total sulfhydryl groups were also altered upon CP treatment indicating the generation of oxidative stress. The maximum changes in all the parameters studied above were 3 days after administration of CP and then recovery took place on days 5 and 7. Thus, the administration of CP leads to significant alterations in the activities of BBM enzymes and the anti-oxidant status of rat intestine. (c) 2007 Elsevier Inc. All rights reserved.
机译：顺铂（CP）是一种广泛使用的抗肿瘤药，具有胃肠道毒性。目前的工作是为了研究CP对大鼠肠刷缘膜（BBM）酶和抗氧化系统的影响。给雄性Wistar大鼠单次腹膜内给予CP（6 mg / kg体重），然后在该治疗后第1、3、5和7天处死。对照动物仅给予盐水。 CP的施用导致粘膜匀浆和分离的膜囊泡中的BBM酶的比活性显着下降。动力学研究表明，CP处理大鼠的BBM囊泡中酶的V-max降低，而K-m保持不变。与对照组相比，CP处理的动物中过氧化氢酶，铜锌超氧化物歧化酶，6-磷酸葡萄糖脱氢酶和谷胱甘肽还原酶的活性降低，而谷胱甘肽S-转移酶和硫氧还蛋白还原酶的活性增加。 CP处理后脂质过氧化和总巯基也发生了变化，表明氧化应激的产生。以上研究的所有参数的最大变化是在CP给药后3天，然后在第5天和第7天恢复。因此，CP给药导致BBM酶的活性和抗氧化剂状态的显着改变。大鼠肠。 （c）2007 Elsevier Inc.保留所有权利。

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