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首页> 外文期刊>Life sciences >Microarray analysis reveals influence of the sesquiterpene lactone parthenolide on gene transcription profiles in human epithelial cells.
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Microarray analysis reveals influence of the sesquiterpene lactone parthenolide on gene transcription profiles in human epithelial cells.

机译：微阵列分析揭示了倍半萜内酯单酚对人上皮细胞基因转录谱的影响。

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摘要

Sesquiterpene lactones are known for their anti-inflammatory activity which has been proven in various assays on DNA, mRNA and protein level. Here we report on the change in the gene expression profile in TNF-alpha stimulated human 293 cells after treatment with parthenolide using a cDNA microarray analysis. Twenty-one of 7028 genes were found to be up- and 18 down-regulated. They encode for chemoattractants, immune system proteins, glycoproteins, metabolism, serine proteinases, and transcription factors. Confirmatory analyses were carried out using quantitative real-time RT-PCR (TaqMan). Additional studies with selected genes revealed the concentration-dependent influence of parthenolide on the expression of these genes.
机译：倍半萜内酯具有抗炎活性，已在DNA，mRNA和蛋白质水平的各种测定中得到证实。在这里，我们报道了用单粉酚使用cDNA微阵列分析治疗后，TNF-α刺激的人293细胞中基因表达谱的变化。发现7028个基因中有21个被上调，而18个被下调。它们编码趋化因子，免疫系统蛋白，糖蛋白，代谢，丝氨酸蛋白酶和转录因子。使用定量实时RT-PCR（TaqMan）进行验证性分析。对选定基因的其他研究表明，小白菊内酯对这些基因的表达具有浓度依赖性。

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