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Effect of ivermectin on the cellular and humoral immune responses of rabbits.

机译：伊维菌素对家兔细胞和体液免疫反应的影响。

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The objective of this paper is to determine the effect of ivermectin administration on cell mediated (CMI) and humoral immunity (HI) of rabbits. CMI against dinitrochlorobenzene (DNCB) and sheep red blood cells (SRBC) in rabbits was determined by delayed-type hypersensitivity and macrophage engulfment assay (MEA), respectively; whereas, HI to Pasteurella multocida B2 vaccine and SRBC was determined by indirect haemagglutination assay (IHA) and Jerne hemolytic plaque formation assay (JHPFA), respectively. The rabbits were divided into four major groups (A through D) each subdivided into four sub-groups (1 through 4). Rabbits of group A served as vehicle control while those of groups B, C and D were treated with ivermectin at the dose rates of 200 microg/kg, 400 microg/kg and 600 microg/kg b.w., respectively. Cellular immunity was determined in sub-groups 1 and 2 through DNCB and MEA, respectively while HI was determined in sub-groups 3 and 4 through IHA and JHPFA, respectively. The skin sensitivity to DNCB at 24 and 48 h and macrophage engulfment of SRBC were highest (P>0.05) in rabbits administered with 600 microg/kg b.w. The highest geometric mean titers (14.00+/-0.31) and number of plaque forming units (1860+/-0.75) were found in rabbits that received ivermectin at a dose of 600 microg/kg b.w. followed, in order by the groups that received 400 microg/kg, 200 microg/kg b.w. and controls. Leukocyte counts were significantly higher in ivermectin-treated groups (C and D) than group A (vehicle control) and B (ivermectin at the rate of 200 microg/kg). A graded dose immune response suggested an immunopotentiating effect of ivermectin at higher doses.
机译：本文的目的是确定伊维菌素对家兔的细胞介导（CMI）和体液免疫（HI）的影响。分别通过迟发型超敏反应和巨噬细胞吞噬试验（MEA）测定家兔对二硝基氯苯（DNCB）和绵羊红细胞（SRBC）的CMI;分别通过间接血凝测定（IHA）和耶恩溶血性斑块形成测定（JHPFA）确定多杀性巴氏杆菌B2疫苗和SRBC的HI。将兔子分成四个主要组（A至D），每组又分为四个亚组（1至4）。 A组的兔子用作媒介物对照，而B，C和D组的兔子分别用伊维菌素以200微克/千克，400微克/千克和600微克/千克体重的剂量率处理。通过DNCB和MEA分别在第1和第2组中确定了细胞免疫，而通过IHA和JHPFA分别在第3和第4组中确定了HI。在600微克/千克体重的兔子中，24h和48h对DNCB的皮肤敏感性和SRBC的巨噬细胞吞噬最高（P> 0.05）。在接受伊维菌素剂量为600 microg / kg b.w的兔子中发现最高的几何平均滴度（14.00 +/- 0.31）和噬菌斑形成单位数（1860 +/- 0.75）。其次，按接受体重400微克/公斤，体重200微克/公斤的组。和控件。伊维菌素治疗组（C和D）的白细胞计数显着高于A组（媒介对照组）和B组（伊维菌素的200微克/千克）。分级剂量的免疫反应提示伊维菌素在较高剂量时具有免疫增强作用。

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