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首页> 外文期刊>Life sciences >Proteomic identification of haptoglobin as a stroke plasma biomarker in spontaneously hypertensive stroke-prone rats.
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Proteomic identification of haptoglobin as a stroke plasma biomarker in spontaneously hypertensive stroke-prone rats.

机译：蛋白质组学鉴定触觉珠蛋白作为自发性高血压中风易发大鼠的中风血浆生物标志物。

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AIMS: We investigated changes in the expression of plasma proteins in spontaneously hypertensive stroke-prone rats (SHRSP) to identify stroke biomarkers. MAIN METHODS AND KEY FINDINGS: The present analysis using surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) demonstrated that three peaks at mass/charge ratios (m/z) of 9330, 9480 and 9700 decreased in intensity during the development and progression of hypertensive stroke in SHRSPs, but not in age-matched control SHR and Wistar rats. Administration of verapamil, an L-type calcium channel blocker which was effective for hypertension in SHRSP rats, prevented the decrease in plasma protein expression. A candidate biomarker protein (m/z 9330) was identified using LC-MS/MS as haptoglobin (Hp). Immunoblotting with anti-Hp antibody demonstrated the decreased expression of both Hpalpha and Hpbeta chains in SHRSP. In contrast, haptoglobin mRNA expression in the liver of SHRSPs slightly increased as compared with control rats. SIGNIFICANCE: These findings suggest that Hp is a biomarker candidate for discriminating pathogenic alterations of stroke.
机译：目的：我们调查了自发性高血压卒中易发大鼠（SHRSP）血浆蛋白表达的变化，以鉴定卒中生物标志物。主要方法和主要发现：使用表面增强的激光解吸/电离飞行时间质谱（SELDI-TOF-MS）进行的当前分析表明，质量/电荷比（m / z）的三个峰分别为9330、9480和9700 SHRSPs在高血压性中风的发生和发展过程中的强度变化，但与年龄相匹配的对照SHR和Wistar大鼠却没有变化。服用维拉帕米（一种对SHRSP大鼠高血压有效的L型钙通道阻滞剂）可防止血浆蛋白表达下降。使用LC-MS / MS鉴定了候选生物标志物蛋白（m / z 9330）为触珠蛋白（Hp）。抗Hp抗体的免疫印迹证明SHRSP中Hpalpha和Hpbeta链的表达均降低。相反，与对照大鼠相比，SHRSPs肝中触珠蛋白mRNA的表达略有增加。意义：这些发现表明，Hp是鉴别中风病原性变化的生物标志物候选物。

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