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首页> 外文期刊>Life sciences >Asymmetric regulation by estrogen at the cholinergic gene locus in differentiated NG108-15 neuronal cells.
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Asymmetric regulation by estrogen at the cholinergic gene locus in differentiated NG108-15 neuronal cells.

机译：雌激素在分化的NG108-15神经元细胞中胆碱能基因基因座的不对称调节。

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AIMS: Estrogen acts as a neurogenerative and neuroprotective factor in the cholinergic system. Choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) are regarded as markers of cholinergic neurons. The genes coding these proteins are located at a common locus, the cholinergic gene locus. However, few details concerning activation of the locus have been obtained. We examined the effect of estrogen on the activation pattern of the locus using a cholinergic cell line. MAIN METHODS: NG108-15 neuronal cells, as a model of cholinergic neurons, were used. Dose-dependent effects of estradiol (E2) on the gene expression of ChAT and VAChT were quantitatively determined by a real-time RT-PCR. The expression of ChAT mRNA variants was qualitatively evaluated by RT-PCR using specific primers. KEY FINDINGS: The expression of ChAT and VAChT mRNA was strongly enhanced with the induction of differentiation. The enhanced expression of ChAT mRNA was further increased dose-dependently by E2 (10(-10) to 10(-7)M), while that of VAChT mRNA did not respond to E2. The up-regulation of ChAT mRNA expression by E2 was abolished by co-treatment with a pure-antagonist of estrogen receptors. A qualitative analysis of ChAT mRNA variants revealed the R types, which share a common sequence with the VAChT gene, and type M ChAT mRNA to mainly be expressed, and that the appearance of these variants was not altered by E2. SIGNIFICANCE: The cholinergic gene locus in differentiated NG108-15 neuronal cells is further activated by E2, but the effect is restricted to the transcription of ChAT gene.
机译：目的：雌激素在胆碱能系统中起神经生成和神经保护因子的作用。胆碱乙酰转移酶（ChAT）和囊泡乙酰胆碱转运蛋白（VAChT）被认为是胆碱能神经元的标志物。编码这些蛋白质的基因位于一个共同的基因座，即胆碱能基因基因座。但是，很少获得有关基因座激活的细节。我们使用胆碱能细胞系检查了雌激素对基因座激活模式的影响。主要方法：NG108-15神经元细胞，作为胆碱能神经元的模型。通过实时RT-PCR定量测定雌二醇（E2）对ChAT和VAChT基因表达的剂量依赖性作用。使用特定引物通过RT-PCR定性评估ChAT mRNA变体的表达。主要结果：ChAT和VAChT mRNA的表达随着分化的诱导而大大增强。 ChAT mRNA的增强表达被E2进一步剂量依赖性地增加（从10（-10）到10（-7）M），而VAChT mRNA的表达不响应E2。通过与雌激素受体的纯拮抗剂共同处理，消除了E2对ChAT mRNA表达的上调。 ChAT mRNA变体的定性分析显示，R型与VAChT基因具有相同的序列，而M型主要是ChAT mRNA，并且E2不会改变这些变体的外观。意义：分化的NG108-15神经元细胞中的胆碱能基因位点被E2进一步激活，但作用仅限于ChAT基因的转录。

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