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首页> 外文期刊>Life sciences >Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract.
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Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract.

机译：通过口服加强免疫力与卵白茄提取物共同施用，增强卵清蛋白特异性IgA反应。

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摘要

An experiment was conducted with the objective to enhance mucosal immunity against ovalbumin (OVA) by co-administration of OVA with an aqueous extract from the fruit of Solanum torvum (STE). Five groups of female ICR mice aged approximately 8 weeks at the commencement of the experiment were caged in groups of eight and received various treatments. The treatments included OVA alone, OVA with cholera toxin (CT), and OVA with various doses of STE. Mice were primed intraperitoneally with 500 microg of OVA alone or co-administered with 0.1 microg CT, or with 1 microg STE. All mice were boosted orally via gastric intubation 14 days after priming with 10 mg OVA alone, or co-administered with 10 microg CT or with 10 mg, 1 mg or 0.1 mg STE. One week later all mice were killed and organs obtained for analysis of the immune response. Intestinal, faecal and pulmonary OVA-specific sIgA concentration was significantly increased (p<0.05) in mice that received booster combinations of OVA/CT and OVA with all extract doses (p<0.05). Specific serum IgG titres did not differ significantly between groups. It is concluded that STE can significantly enhance secretory immunity in the intestine to OVA with mucosal homing to the lungs. The adjuvant effect of STE is comparable to that of CT.
机译：进行实验的目的是通过与卵白茄果实（STE）的水提取物共同给药来增强对卵清蛋白（OVA）的粘膜免疫力。在实验开始时将大约八周龄的五组雌性ICR小鼠关在笼中，每组八只，并接受各种治疗。这些治疗包括单独的OVA，带有霍乱毒素（CT）的OVA和带有不同剂量的STE的OVA。单独用500微克OVA或与0.1微克CT或1微克STE共同给药对小鼠进行腹膜内灌注。初次用10 mg OVA或与10 microg CT或10 mg，1 mg或0.1 mg STE共同给药后14天，通过胃插管对所有小鼠进行口服加强免疫。一周后，杀死所有小鼠并获得器官用于分析免疫应答。在所有剂量的OVA / CT和OVA加强免疫组合的小鼠中，肠道，粪便和肺的OVA特异性sIgA浓度均显着增加（p <0.05）。两组之间的特异性血清IgG滴度无明显差异。结论是，STE可以通过粘膜归巢到肺部来显着增强肠道对OVA的分泌免疫。 STE的辅助作用与CT相当。

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