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Effect of beta-alanyl-L-histidinato zinc on the differentiation pathway of human periodontal ligament cells

机译：β-丙氨酰-L-组氨酸锌对人牙周膜细胞分化途径的影响

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We examined the effect of beta-alanyl-L-histidinato zinc (AHZ) on the differentiation of human periodontal ligament (HPDL) cells. HPDL cells were cultured with alpha-minimum essential medium containing 10% fetal bovine serum with or without 10(-4) or 10(-5) M AHZ for up to 10 days. The expression of runt-related transcription factor-2/core binding factor alpha-1 (RUNX2/Cbfal), Sox9, bone morphogenetic proteins (BMPs), and BMP receptors was measured using serniquantitative reverse transcription-polymerase chain reactions. Phosphorylation of Smad1 was determined using a Western blot analysis. RUNX2/Cbfal expression increased markedly in cells cultured with AHZ, while Sox9 expression increased slightly. BMP-7 expression was much higher than that of controls in cultures with AHZ, whereas BMP-2 expression was only slightly higher. The expression of BMP receptors increased markedly in cells cultured with AHZ. The phosphorylation of Smad1, a signal-transducing molecule for BMP-2 and BMP-7, was increased markedly in cultures with AHZ. The results suggest that AHZ diverts the differentiation pathway of HPDL cells to the osteoblast lineage via BMP-2 or BMP-7. (C) 2004 Elsevier Inc. All rights reserved. [References: 29]
机译：我们检查了β-丙氨酰-L-组氨酸锌（AHZ）对人牙周膜（HPDL）细胞分化的影响。将HPDL细胞与含有10％胎牛血清，有或没有10（-4）或10（-5）M AHZ的α-最小必需培养基培养10天。矮小相关转录因子2 /核心结合因子α-1（RUNX2 / Cbfal），Sox9，骨形态发生蛋白（BMP）和BMP受体的表达使用反转录聚合酶链反应进行了测量。使用蛋白质印迹分析确定Smad1的磷酸化。在AHZ培养的细胞中，RUNX2 / Cbfal表达显着增加，而Sox9表达则略有增加。在具有AHZ的培养物中，BMP-7表达远高于对照，而BMP-2表达仅略高。在用AHZ培养的细胞中，BMP受体的表达显着增加。 Smad1是BMP-2和BMP-7的信号转导分子，在AHZ培养物中的磷酸化显着增加。结果表明AHZ通过BMP-2或BMP-7将HPDL细胞的分化途径转移至成骨细胞谱系。 （C）2004 Elsevier Inc.保留所有权利。 [参考：29]

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