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首页> 外文期刊>Life sciences >SLC5A9/SGLT4, a new Na(+)-dependent glucose transporter, is an essential transporter for mannose, 1,5-anhydro-D-glucitol, and fructose.
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SLC5A9/SGLT4, a new Na(+)-dependent glucose transporter, is an essential transporter for mannose, 1,5-anhydro-D-glucitol, and fructose.

机译：SLC5A9 / SGLT4是一种新的依赖Na（+）的葡萄糖转运蛋白，是甘露糖，1,5-脱水D-葡萄糖醇和果糖的必需转运蛋白。

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摘要

We isolated a cDNA clone of SLC5A9/SGLT4 from human small intestinal full-length cDNA libraries, and functionally characterized it in vitro. The messenger RNA encoding SGLT4 was mainly expressed in the small intestine and kidney, among the human tissues tested. COS-7 cells transiently expressing SGLT4 exhibited Na(+)-dependent alpha-methyl-D-glucopyranoside (AMG) transport activity with an apparent K(m) of 2.6 mM, suggesting that SGLT4 is a low affinity-type transporter. The rank order of naturally occurring sugar analogs for the inhibition of AMG transport was: D-mannose (Man) D-glucose (Glc) > D-fructose (Fru) = 1,5-anhydro-D-glucitol (1,5AG) > D-galactose (Gal). Recognition of Man as a substrate was confirmed by direct uptake of Man into the cell. COS-7 cells expressing a putative murine SGLT4 ortholog showed similar Na(+)-dependent AMG transport activity and a similar deduced substrate specificity. These results suggest that SGLT4 would have unique physiological functions (i.e., absorption and/or reabsorption of Man, 1,5AG, and Fru, in addition to Glc).
机译：我们从人小肠全长cDNA文库中分离了SLC5A9 / SGLT4的cDNA克隆，并在体外对其功能进行了表征。在测试的人体组织中，编码SGLT4的信使RNA主要在小肠和肾脏中表达。瞬时表达SGLT4的COS-7细胞具有Na（+）依赖性的α-甲基-D-吡喃葡萄糖苷（AMG）转运活性，其表观K（m）为2.6 mM，表明SGLT4是一种低亲和力的转运蛋白。用于抑制AMG转运的天然糖类似物的排序顺序为：D-甘露糖（Man） D-葡萄糖（Glc）> D-果糖（Fru）= 1,5-脱水-D-葡萄糖醇（1， 5AG）> D-半乳糖（Gal）。通过将Man直接摄取到细胞中来确认将Man识别为底物。表达假定鼠SGLT4直系同源物的COS-7细胞显示相似的Na（+）依赖性AMG转运活性和相似的推定底物特异性。这些结果表明，SGLT4将具有独特的生理功能（即，除了Glc之外，Man，1,5AG和Fru的吸收和/或重吸收）。

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