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首页> 外文期刊>Life sciences >Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone.
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Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone.

机译：搪瓷基质衍生物是乳腺癌细胞附着在骨上的有效抑制剂。

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This study examined whether enamel matrix derivative (EMD) inhibits the adhesion of cancer cells to bone. A typical breast cancer cell line, MCF-7, was used. Conditioned human osteosarcoma cell (Saos-2) medium was used as extracellular bone matrix (ECBM) to measure cell attachment. MCF-7 cells were incubated on ECBM-coated culture plates with or without soluble EMD, Arg-Gly-Asp (RGD) sequence blocking peptides, recombinant bone sialoprotein (rBSP), or specific integrin antibodies, and the attached cells were quantified using toluidine blue staining. EMD markedly reduced the attachment of MCF-7 cells to ECBM in a dose-dependent manner. An RGD peptide (GRGDSP) and recombinant BSP inhibited cell attachment to the same degree as EMD. Similarly, anti-alphavbeta3 integrin antibody strongly reduced cell attachment, whereas anti-alphavbeta5 and anti-beta1 integrin antibodies had less marked effects on cell attachment. These results show that EMD inhibits MCF-7 cell attachment to a bone matrix and that it mightbe useful as an anti-adhesive agent for breast cancer cells to bone in vivo.
机译：这项研究检查了釉质基质衍生物（EMD）是否抑制癌细胞对骨骼的粘附。使用了典型的乳腺癌细胞系MCF-7。条件人类骨肉瘤细胞（Saos-2）培养基用作细胞外骨基质（ECBM）来测量细胞附着。将MCF-7细胞在带有或不带有可溶性EMD，Arg-Gly-Asp（RGD）序列阻断肽，重组骨唾液蛋白（rBSP）或特定整合素抗体的ECBM包被的培养板上孵育，并使用甲苯胺对附着的细胞进行定量蓝色染色。 EMD以剂量依赖性方式显着降低了MCF-7细胞对ECBM的附着。 RGD肽（GRGDSP）和重组BSP抑制细胞附着的程度与EMD相同。同样，抗-αvbeta3整合素抗体强烈降低细胞粘附，而抗-αvbeta5和抗-β1整合素抗体对细胞粘附的影响较小。这些结果表明，EMD抑制MCF-7细胞附着在骨基质上，并可能用作乳腺癌细胞在体内与骨骼的抗粘连剂。

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