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首页> 外文期刊>Life sciences >Regulatory mechanism of Toona sinensis on mouse leydig cell steroidogenesis.
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Regulatory mechanism of Toona sinensis on mouse leydig cell steroidogenesis.

机译：香椿对小鼠leydig细胞类固醇生成的调控机制。

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摘要

Toona sinensis (TS), a kind of arbor, widely distributes nowadays in Asia. The leaves of TS have been used as an effective nutritious food in Chinese society for a long time. It was reported that Toona sinensis can induce apoptosis of cancer cells, reduce plasma glucose in diabetic rats, and improve lipolysis of differentiated 3T3-L1 adipocyte and its uptake of glucose. It has also been shown that TS may increase dynamic activity of human sperm. Thus, we are interested to investigate whether Toona sinensis has any effect on mouse Leydig cell testosterone production, which correlates to sperm activity. Primary mouse Leydig cells were purified to conduct the in vitro experiments. Different concentrations of crude Toona sinensis were added to primary mouse Leydig cells and the testosterone production was determined. The results showed that crude TS significantly inhibited both basal and human chorionic gonadotropin (hCG)-stimulated testosterone productions in dose dependent manner, respectively (P<0.05). Crude TS also reduced the forskolin- and dibutyryl-cAMP (dbcAMP)-stimulated testosterone production (P<0.05), which indicated that crude TS might affect protein kinase A (PKA) signal transduction pathway at the site after the formation of cyclic AMP. Moreover, TS inhibited Leydig cell steroidogenesis by suppressing the activity of steroidogenic enzymes including P450 side chain cleavage enzyme, 3 beta-hydroxysteroid dehydrogenase, 17 alpha-hydroxylase, 20 alpha-hydroxylase and 17 beta-hydroxysteroid dehydrogenase (P<0.05). In summary, these results suggested that TS inhibited steroidogenesis by suppressing the cAMP-PKA signaling pathway and the activities of steroidogenic enzymes in normal mouse Leydig cells.
机译：香椿（Toona sinensis，TS）是一种乔木，如今在亚洲广泛分布。长期以来，TS的叶子一直被用作中国社会的有效营养食品。据报道，香椿可以诱导糖尿病大鼠的癌细胞凋亡，降低血浆葡萄糖，并改善分化的3T3-L1脂肪细胞的脂解作用及其对葡萄糖的吸收。还已经表明TS可以增加人类精子的动态活性。因此，我们有兴趣研究香椿是否对小鼠睾丸间质细胞睾丸激素产生有任何影响，这与精子活性有关。纯化原代小鼠Leydig细胞以进行体外实验。将不同浓度的粗制香椿加到原代小鼠Leydig细胞中，测定睾丸激素的产生。结果表明，粗制TS分别以剂量依赖方式显着抑制基础和人绒毛膜促性腺激素（hCG）刺激的睾丸激素的产生（P <0.05）。粗制TS还减少了福司可林和二丁酰-cAMP（dbcAMP）刺激的睾丸激素的产生（P <0.05），这表明粗制TS可能会在环状AMP形成后影响该部位的蛋白激酶A（PKA）信号转导途径。此外，TS通过抑制类固醇生成酶（包括P450侧链裂解酶，3个β-羟基类固醇脱氢酶，17个α-羟基化酶，20个α-羟基化酶和17个β-羟基类固醇脱氢酶）的活性来抑制莱伊迪格细胞类固醇生成（P <0.05）。总之，这些结果表明，TS通过抑制cAMP-PKA信号通路和正常小鼠Leydig细胞中类固醇生成酶的活性来抑制类固醇生成。

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