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首页> 外文期刊>Life sciences >Stereoselective effects of 3-hydroxybutyrate on glucose utilization of rat cardiomyocytes.
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Stereoselective effects of 3-hydroxybutyrate on glucose utilization of rat cardiomyocytes.

机译：3-羟基丁酸酯对大鼠心肌细胞葡萄糖利用的立体选择性作用。

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In researches of ketone bodies, D-3-hydroxybutyrate (D-3HB) is usually the major one which has been investigated; in contrast, little attention has been paid to L-3-hydroxybutyrate (L-3HB), because of its presence in trace amounts, its dubious metabolism, and a lack of knowledge about its sources. In the present study we determined the distributions of enantiomers of 3-hydroxybutyrate (3HB) in rat brain, liver, heart, and kidney homogenates, and we found the heart homogenate contained an enriched amount of L-3HB (37.67 microM/mg protein) which generated a significant ratio of 66/34 (D/L). The ratio was altered to be 87/13 in the diabetic rat heart homogenate. We subsequently found this changed ratio of D/L-3HB may contribute to reduce glucose utilization in cardiomyocytes. Glucose utilization by cardiomyocytes with 5 mM of D-3HB was decreased to 61% of the control, but no interference was observed when D-3HB was replaced with L-3HB, suggesting L-3HB is not utilized for the energy fuel as other ketone bodies are. In addition, the reduced glucose utilization caused by D-3HB gradually recovered in a dose-dependent manner with administration of additional L-3HB. The results gave the necessity of taking L-3HB together with D-3HB into account with regard to glucose utilization, and L-3HB may be a helpful substrate for improving inhibited cardiac pyruvate oxidation caused by hyperketonemia.
机译：在酮体的研究中，D-3-羟基丁酸酯（D-3HB）通常是主要研究对象。相比之下，由于L-3-羟基丁酸酯（L-3HB）的存在量少，其可疑的新陈代谢以及缺乏有关其来源的知识，因此几乎未引起注意。在本研究中，我们确定了3-羟基丁酸酯（3HB）对映异构体在大鼠脑，肝，心脏和肾脏匀浆中的分布，我们发现心脏匀浆中含有丰富的L-3HB（37.67 microM / mg蛋白）产生显着的比率66/34（D / L）。在糖尿病大鼠心脏匀浆中，该比例更改为87/13。我们随后发现D / L-3HB的这种变化比例可能有助于减少心肌细胞中的葡萄糖利用。 D-3HB浓度为5 mM的心肌细胞对葡萄糖的利用率降低至对照组的61％，但当D-3HB替换为L-3HB时未观察到干扰，这表明L-3HB未作为能源与其他酮一起使用身体。另外，由D-3HB引起的降低的葡萄糖利用率通过施用另外的L-3HB以剂量依赖性方式逐渐恢复。结果表明，在葡萄糖利用方面必须考虑将L-3HB与D-3HB一起使用，并且L-3HB可能是改善由高血钾引起的心脏丙酮酸氧化抑制的有用底物。

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