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首页> 外文期刊>Cell biology international. >Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells
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Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells

机译：通过将嗜热四膜虫和鱼细胞共培养来描绘纤毛虫和鱼之间的细胞相互作用

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摘要

Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant for phagocytosis) were cultured on monolayers of 3 fish epithelial cell lines, CHSE-214, RTgill-W1, and ZEB2J, and the rabbit kidney epithelial cell line, RK-13. Generally the ciliates flourished, whereas the monolayers died, being completely consumed over several days. The destruction of monolayers reqviired that the ciliates could make contact with the animal cells through swimming, which appeared to dislodge or loosen cells so that they could be phagocytosed. The ciliates internalized into food vacuoles ZEB2J from cell monolayers as well as from cell suspensions. Phagocytosis was essential for monolayer destruction as monolayers remained intact under conditions where phagocytosis was impeded, such as 37°C for NP1 and 4°C for B1975. Monolayers offish cells supported the proliferation of ciliates. Thus T. thermophila can 'eat' animal cells or be histophagous in vitro, with the potential to be histophagous in vivo.
机译：尽管四膜虫的几种物种通常被描述为鱼类的噬菌体和机会病原体，但对纤毛虫/​​鱼类细胞相互作用的了解甚少，但研究这些的一种方法是在体外用细胞系进行研究。在这项研究中，嗜热衣原体，B1975（野生型）和NP1（吞噬作用的温度敏感突变体）在3种鱼上皮细胞系CHSE-214，RTgill-W1和ZEB2J以及兔肾上皮细胞的单层上培养线，RK-13​​。通常，纤毛蓬勃发展，而单层细胞死亡，在数天内被完全消耗。单层细胞的破坏要求纤毛虫可以通过游泳与动物细胞接触，从而使纤毛看起来脱落或松弛，从而可以吞噬它们。纤毛从细胞单层以及从细胞悬液内化到食物液泡ZEB2J中。吞噬作用对于单层破坏至关重要，因为在阻止吞噬作用的条件下，例如NP1的37°C和B1975的4°C，单层仍然保持完整。鱼细胞的单层支持纤毛虫的增殖。因此，嗜热毁丝菌可以在体外“吞噬”动物细胞或使其成为噬菌体，而在体内则有可能成为噬菌体。

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