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首页> 外文期刊>Life sciences >SPECIFIC BINDING OF [H-3]RESINIFERATOXIN BY HUMAN AND RAT PREOPTIC AREA, LOCUS CERULEUS, MEDIAL HYPOTHALAMUS, RETICULAR FORMATION AND VENTRAL THALAMUS MEMBRANE PREPARATIONS
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SPECIFIC BINDING OF [H-3]RESINIFERATOXIN BY HUMAN AND RAT PREOPTIC AREA, LOCUS CERULEUS, MEDIAL HYPOTHALAMUS, RETICULAR FORMATION AND VENTRAL THALAMUS MEMBRANE PREPARATIONS

机译：人和大鼠视前区，粘液蓝斑，内侧下丘脑，网状结构和腹侧丘脑膜制备物对[H-3]视铁蛋白毒素的特定结合

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Specific [H-3]resiniferatoxin (RTX) binding detects the vanilloid (capsaicin) receptors and provides a biochemical means for exploring their pharmacology. In the present study we demonstrate specific vanilloid (RTX) binding sites in various brain areas not known to be innervated by primary afferent neurons. Specific high-affinity binding of [H-3]RTX could be detected in membrane preparations of the posterior (''hypothalamic'') and anterior (''septal'') parts of the preoptic area, locus ceruleus, medial hypothalamus, brainstem reticular formation and ventral thalamic nuclei from naive rats. The determined levels of binding at 4 nM [H-3]RTX were 23.0 +/- 4.5, 7.1 +/- 1.6, 29.9 +/- 2.3, 23.5 +/- 2.4, 9.9 +/- 2.2 and 8.1 +/- 1.9 fmol/mg, respectively; unfortunately, the high levels of non-specific binding (higher than 80 %) in the present experiments made it impossible for us to characterize fully the binding properties of the receptors. However, no detectable specific [H-3]RTX binding was present in membranes of brain nuclei from rats pretreated with 300 mg/kg capsaicin, a treatment which causes loss of response to capsaicin. Significant specific [H-3]RTX binding was also absent in membrane preparations of the midbrain central gray matter, somatosensory cortex and cerebellum either from naive or capsaicin treated rats. In human brain specific [H-3]RTX binding measured at 4 nM [H-3]RTX showed a pattern of distribution similar to that in the rat brain. The corresponding levels of specific [H-3]RTX binding in the preoptic area, locus ceruleus, medial hypothalamus, reticular formation and ventral thalamus were 44.9 +/- 2.4, 50.6 +/- 3.0, 36.1 +/- 2.9, 9.4 +/- 2.8 and 8.4 +/- 2.4 fmol/mg, respectively. Our findings corroborate previous biological evidence that vanilloid receptors are present in brain as well as in sensory afferent neurons. [References: 55]
机译：特定的[H-3]树脂毒素（RTX）结合可以检测类香草素（辣椒素）受体，并提供一种生化手段来探索其药理作用。在本研究中，我们证明了在未知被初级传入神经元支配的各个大脑区域中的特定类香草酸（RTX）结合位点。 [H-3] RTX的特异性高亲和力结合可以在视前区，下丘脑，下丘脑内侧，脑干的后部（“下丘脑”）和前部（“中隔”）的膜制品中检测到幼鼠的网状结构和腹侧丘脑核。在4 nM [H-3] RTX处测定的结合水平为23.0 +/- 4.5、7.1 +/- 1.6、29.9 +/- 2.3、23.5 +/- 2.4、9.9 +/- 2.2和8.1 +/- 1.9 fmol / mg;不幸的是，在本实验中高水平的非特异性结合（高于80％）使我们无法充分表征受体的结合特性。然而，在用300 mg / kg辣椒素预处理的大鼠的脑核膜上没有可检测到的特异性[H-3] RTX结合，该处理导​​致辣椒素反应丧失。在幼稚或辣椒素处理的大鼠的中脑中央灰质，体感皮层和小脑的膜制剂中也没有明显的[H-3] RTX特异性结合。在人脑中，在4 nM下测量的[H-3] RTX特异性结合[H-3] RTX显示出与大鼠脑相似的分布模式。视前区，蓝斑，下丘脑内侧，网状结构和腹侧丘脑的特定[H-3] RTX结合水平分别为44.9 +/- 2.4、50.6 +/- 3.0、36.1 +/- 2.9、9.4 + / -分别为2.8和8.4 +/- 2.4 fmol / mg。我们的发现证实了以前的生物学证据，即在大脑以及感觉传入神经元中均存在类香草酸受体。 [参考：55]

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